Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   LAJ50_RS13745 Genome accession   NZ_CP083376
Coordinates   3077465..3077869 (+) Length   134 a.a.
NCBI ID   WP_138652516.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. X-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3072465..3082869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ50_RS13730 (LAJ50_13720) sucC 3072579..3073748 (-) 1170 WP_130549733.1 ADP-forming succinate--CoA ligase subunit beta -
  LAJ50_RS13735 (LAJ50_13725) - 3073934..3075562 (+) 1629 WP_130549734.1 ATP-binding protein -
  LAJ50_RS13740 (LAJ50_13730) pilR 3075653..3077122 (+) 1470 WP_130549735.1 sigma-54 dependent transcriptional regulator Regulator
  LAJ50_RS13745 (LAJ50_13735) pilA2 3077465..3077869 (+) 405 WP_138652516.1 pilin Machinery gene
  LAJ50_RS13750 (LAJ50_13740) - 3077978..3079690 (+) 1713 WP_138652514.1 hypothetical protein -
  LAJ50_RS13755 (LAJ50_13745) pilB 3079755..3081479 (+) 1725 WP_138652512.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13897.01 Da        Isoelectric Point: 9.4342

>NTDB_id=605450 LAJ50_RS13745 WP_138652516.1 3077465..3077869(+) (pilA2) [Pseudoxanthomonas sp. X-1]
MKKNMQGFTLIELMIVVAIIAILAAIALPAYSDYTKKAKVSEVILAASSARTAVAEYAAGNNTLPPTTWAPEVQSSKYVS
GLAWSGGKIVATSTVPGATGNITLTPTLNTANSQVTWVCDGSVSSKYRPGTCQG

Nucleotide


Download         Length: 405 bp        

>NTDB_id=605450 LAJ50_RS13745 WP_138652516.1 3077465..3077869(+) (pilA2) [Pseudoxanthomonas sp. X-1]
ATGAAGAAGAACATGCAGGGCTTCACCCTCATCGAACTGATGATCGTGGTCGCCATCATCGCCATCCTGGCCGCCATCGC
GCTGCCGGCGTACTCCGATTACACCAAGAAGGCCAAGGTCTCCGAAGTCATTCTCGCGGCTTCCTCTGCTCGTACTGCAG
TTGCCGAGTACGCCGCTGGAAACAACACTCTGCCCCCGACCACTTGGGCGCCGGAAGTTCAGTCGTCCAAGTATGTCTCC
GGACTTGCATGGAGTGGCGGCAAGATCGTTGCCACCAGTACTGTGCCGGGCGCGACCGGCAACATCACGCTCACCCCGAC
TCTTAACACGGCAAATTCTCAAGTCACCTGGGTCTGTGATGGCTCTGTTTCTTCCAAGTATCGCCCGGGCACCTGCCAAG
GCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

49.275

100

0.507
  pilA2 Legionella pneumophila str. Paris

49.624

99.254

0.493
  comP Acinetobacter baylyi ADP1

44.828

100

0.485
  pilA Ralstonia pseudosolanacearum GMI1000

34.911

100

0.44
  pilE Neisseria gonorrhoeae MS11

36.774

100

0.425
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

100

0.418
  pilE Neisseria gonorrhoeae strain FA1090

34.177

100

0.403
  pilA Glaesserella parasuis strain SC1401

36.986

100

0.403
  pilA/pilA1 Eikenella corrodens VA1

34.868

100

0.396
  pilA Haemophilus influenzae 86-028NP

38.519

100

0.388