Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LAJ50_RS13755 Genome accession   NZ_CP083376
Coordinates   3079755..3081479 (+) Length   574 a.a.
NCBI ID   WP_138652512.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. X-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3074755..3086479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ50_RS13740 (LAJ50_13730) pilR 3075653..3077122 (+) 1470 WP_130549735.1 sigma-54 dependent transcriptional regulator Regulator
  LAJ50_RS13745 (LAJ50_13735) pilA2 3077465..3077869 (+) 405 WP_138652516.1 pilin Machinery gene
  LAJ50_RS13750 (LAJ50_13740) - 3077978..3079690 (+) 1713 WP_138652514.1 hypothetical protein -
  LAJ50_RS13755 (LAJ50_13745) pilB 3079755..3081479 (+) 1725 WP_138652512.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LAJ50_RS13760 (LAJ50_13750) pilC 3081679..3082941 (+) 1263 WP_138652510.1 type II secretion system F family protein Machinery gene
  LAJ50_RS13765 (LAJ50_13755) - 3082945..3083808 (+) 864 WP_130549744.1 A24 family peptidase -
  LAJ50_RS13770 (LAJ50_13760) - 3084056..3085060 (-) 1005 WP_138652508.1 class I fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62739.99 Da        Isoelectric Point: 6.2656

>NTDB_id=605451 LAJ50_RS13755 WP_138652512.1 3079755..3081479(+) (pilB) [Pseudoxanthomonas sp. X-1]
MTLAPAPNLVGVTGIARRLVMDGAMDEATARSVMTDAFQAKIPLSQWIHEKKLVTPSQLAAANAIEFGIPLIDASSFDPS
QNALKLVSEELLQKHQVLPMFKRGNKLYVGVSDPTRMEGLDEIKFHTNLVVEPILVAEDQLRRTLETWTNASDSLAGMGG
DEEDFGSLDTAGGDEESSDTGIDAKGEDTPVVKFVNKMLVDAIRRGASDIHFEPYETDYRVRLRIDGILKTVARAPQKLN
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPRCKRPHDIPPHAL
LSEGFTQADLDAGLTLYQAVGCDECTEGYKGRVGVYQVMPMTDEIAAIVLEGGGAIQIAEAAQRSGIRDLRQSALWKAKQ
GLTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=605451 LAJ50_RS13755 WP_138652512.1 3079755..3081479(+) (pilB) [Pseudoxanthomonas sp. X-1]
ATGACTCTTGCTCCAGCTCCCAATCTTGTCGGTGTAACGGGCATCGCACGGCGTCTAGTGATGGATGGCGCGATGGATGA
AGCAACCGCACGCTCGGTCATGACCGATGCATTCCAAGCGAAGATCCCCTTGTCACAATGGATCCACGAGAAGAAGCTTG
TCACTCCATCGCAATTAGCAGCGGCCAACGCCATCGAGTTCGGCATTCCCCTAATCGACGCGTCGTCATTTGACCCCAGC
CAGAACGCCTTGAAACTGGTAAGTGAAGAACTGTTGCAAAAGCACCAGGTTCTTCCGATGTTCAAACGCGGCAACAAGCT
TTATGTAGGCGTTTCGGACCCTACACGCATGGAGGGGCTGGACGAAATCAAGTTCCACACTAATTTGGTCGTGGAGCCTA
TCTTGGTAGCCGAGGACCAACTCCGCCGCACTTTAGAGACGTGGACTAATGCTTCCGACTCACTCGCTGGCATGGGTGGC
GACGAAGAGGATTTTGGCAGCCTAGACACTGCGGGCGGGGATGAAGAGAGCAGCGACACCGGGATCGACGCCAAAGGCGA
GGACACGCCGGTCGTGAAGTTCGTGAACAAGATGTTGGTCGACGCGATTCGCCGCGGCGCCTCGGATATTCATTTCGAGC
CTTACGAGACGGACTATCGCGTCCGTCTGCGCATCGACGGCATCCTCAAGACCGTCGCGCGCGCGCCACAGAAGCTTAAC
CAGCGCATCGCCGCGCGCCTGAAGGTCATGTCGCAGTTGGATATCGCTGAAAAGCGCGTACCGCAGGACGGCCGCATCAA
ACTCAATCTGTCCAAAACACGTCAGATCGACTTCCGCGTCAGCACCTTGCCGACGCTGTTCGGCGAAAAGGTCGTGCTGC
GTATCCTGGATGGCAGCGCAGCCAAACTGGGCATCGACAAGCTGGGCTATGAGCCGGACCAACAAAAGCTGTTCCTCGAC
GCGATCCACAAGCCGTACGGCATGGTGCTGGTCACCGGTCCGACCGGTTCGGGCAAGACCGTATCGCTGTATACGGCCCT
GGGGATCCTCAACGACGACACCCGCAACATCTCCACGGCGGAGGACCCGGTCGAAATCCGCCTGCCCGGCGTCAACCAAG
TGCAACAGAACAACAAGCGCGGAATGACATTTGCCGCGGCGCTGCGCTCGTTCCTGCGTCAGGATCCGGATATCATCATG
GTCGGCGAAATCCGCGACTTGGAGACGGCCGAGATCGCGGTCAAGGCCGCCCAGACCGGTCACATGGTGCTTTCTACCCT
GCATACCAACGACGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATCGCCCCGTACAACATCACCTCGTCGGTGA
CGCTGGTAATAGCCCAGCGCCTGGCACGCCGCCTTTGCCCACGCTGCAAGCGCCCTCACGACATCCCGCCACATGCCCTG
CTGTCGGAAGGCTTTACCCAAGCCGATCTTGACGCGGGCCTGACCCTTTACCAGGCGGTTGGCTGCGACGAGTGCACGGA
AGGCTACAAGGGGCGCGTAGGTGTCTACCAAGTCATGCCGATGACGGACGAGATTGCCGCGATCGTGCTGGAGGGCGGCG
GCGCAATCCAGATCGCCGAGGCCGCCCAGCGTTCCGGCATCCGCGACCTGCGCCAGTCGGCGCTCTGGAAGGCCAAGCAG
GGATTGACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-147)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.184

98.606

0.554
  pilB Acinetobacter baylyi ADP1

53.659

100

0.537
  pilB Legionella pneumophila strain ERS1305867

52.837

98.258

0.519
  pilF Neisseria gonorrhoeae MS11

51.81

91.463

0.474
  pilB Vibrio cholerae strain A1552

52.745

88.85

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.942

96.864

0.455
  pilB Vibrio campbellii strain DS40M4

48.431

88.85

0.43
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.307

90.767

0.366