Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NI384_RS02905 Genome accession   NZ_CP099928
Coordinates   591642..592085 (-) Length   147 a.a.
NCBI ID   WP_149151214.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain BM24B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586642..597085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI384_RS02885 (NI384_02885) coaE 587154..587768 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  NI384_RS02890 (NI384_02890) pilD 587769..588638 (-) 870 WP_025533026.1 A24 family peptidase Machinery gene
  NI384_RS02895 (NI384_02895) pilC 588703..589926 (-) 1224 WP_308385409.1 type II secretion system F family protein Machinery gene
  NI384_RS02900 (NI384_02900) pilB 589950..591635 (-) 1686 WP_308385410.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI384_RS02905 (NI384_02905) pilA 591642..592085 (-) 444 WP_149151214.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NI384_RS02910 (NI384_02910) nadC 592349..593236 (-) 888 WP_025523107.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI384_RS02915 (NI384_02915) ampD 593329..593880 (+) 552 WP_025536234.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI384_RS02920 (NI384_02920) pdhR 594286..595053 (+) 768 WP_308385411.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15550.88 Da        Isoelectric Point: 10.1101

>NTDB_id=603587 NI384_RS02905 WP_149151214.1 591642..592085(-) (pilA) [Vibrio parahaemolyticus strain BM24B]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAIAIPAYQNYVKKSEAATGLATAKSLITNIDMHIQEVGAFPTNNQAGFTAVG
ATSGMNKLGTLSFESISGANGTIKFLFGNTSSINTAFVEIAKSNSGWACTFTTNNKLTSNEVPKACR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=603587 NI384_RS02905 WP_149151214.1 591642..592085(-) (pilA) [Vibrio parahaemolyticus strain BM24B]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCAATTATTGGGGTGTT
ATCAGCTATCGCGATTCCAGCTTATCAAAACTATGTAAAGAAGTCAGAAGCAGCTACTGGGTTGGCAACCGCAAAATCTC
TTATCACTAATATAGATATGCATATTCAAGAAGTAGGTGCTTTCCCGACTAATAATCAAGCGGGCTTTACTGCTGTTGGT
GCAACGTCAGGGATGAACAAACTTGGCACTCTATCATTCGAGAGTATCAGCGGTGCAAATGGAACCATTAAGTTCCTATT
TGGTAATACTTCTTCTATAAACACGGCATTTGTAGAAATTGCCAAGTCAAATAGCGGGTGGGCATGTACATTTACAACTA
ATAACAAATTAACAAGCAATGAAGTACCAAAAGCTTGTAGATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(41-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.695

100

0.469
  pilA Vibrio cholerae strain A1552

45.695

100

0.469
  pilA Vibrio cholerae C6706

45.695

100

0.469
  pilA Vibrio parahaemolyticus RIMD 2210633

50.769

88.435

0.449
  pilA Acinetobacter baumannii strain A118

42.657

97.279

0.415
  pilA2 Legionella pneumophila strain ERS1305867

38.621

98.639

0.381
  comP Acinetobacter baylyi ADP1

35

100

0.381
  pilA/pilA1 Eikenella corrodens VA1

33.133

100

0.374
  pilA2 Legionella pneumophila str. Paris

37.931

98.639

0.374
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.732

96.599

0.374
  pilA Pseudomonas aeruginosa PAK

35.099

100

0.361