Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   DVS74_RS10510 Genome accession   NZ_CP099516
Coordinates   2288505..2288945 (+) Length   146 a.a.
NCBI ID   WP_012338104.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain ESVL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2283505..2293945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVS74_RS10485 (DVS74_010475) coaE 2283594..2284217 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  DVS74_RS10490 (DVS74_010480) pilD 2284229..2285092 (-) 864 WP_004084594.1 prepilin peptidase Machinery gene
  DVS74_RS10495 (DVS74_010485) pilC 2285099..2286301 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  DVS74_RS10500 (DVS74_010490) pilA 2286742..2287188 (+) 447 WP_128283976.1 pilin Machinery gene
  DVS74_RS10505 (DVS74_010495) - 2287433..2287660 (-) 228 WP_012338102.1 hypothetical protein -
  DVS74_RS10510 (DVS74_010500) pilA/pilAI 2288505..2288945 (+) 441 WP_012338104.1 pilin Machinery gene
  DVS74_RS10515 (DVS74_010505) pilB 2289785..2291518 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DVS74_RS10520 (DVS74_010510) - 2291612..2293024 (-) 1413 WP_020851962.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14979.22 Da        Isoelectric Point: 8.1165

>NTDB_id=602178 DVS74_RS10510 WP_012338104.1 2288505..2288945(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain ESVL]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVAAALAEITPGRTQAEIRMADGQASNTPNAIGLRTPTARCSL
VTVDITTTGGTIVCTMVGNGQVNNQTITLTRIADNNAGQGGVNTGGNWTCTTTAPAALTPAGCTGT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=602178 DVS74_RS10510 WP_012338104.1 2288505..2288945(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain ESVL]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCCGCGATCGCTCT
GCCGATGTATCAGAATTATGTCGCCAGGTCTCAAGTCGCAGCGGCGCTGGCCGAGATCACACCGGGCCGAACACAAGCCG
AAATCCGTATGGCTGATGGACAGGCTTCAAACACTCCTAACGCTATTGGATTACGCACCCCGACCGCTCGTTGCAGCCTC
GTTACGGTCGACATCACAACTACTGGCGGCACAATCGTATGTACGATGGTTGGTAATGGTCAGGTCAACAATCAAACTAT
CACCTTGACCCGCATCGCTGATAACAACGCAGGCCAAGGTGGCGTCAATACTGGGGGGAACTGGACCTGTACCACAACCG
CGCCTGCAGCGTTAACTCCAGCAGGCTGCACTGGAACCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.578

100

0.459
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.466

100

0.425
  pilA Vibrio cholerae strain A1552

38.509

100

0.425
  pilA Vibrio cholerae C6706

38.509

100

0.425
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.509

100

0.425
  pilA Pseudomonas aeruginosa PAK

38.365

100

0.418
  pilA Acinetobacter baumannii strain A118

39.865

100

0.404
  pilA Vibrio parahaemolyticus RIMD 2210633

38.621

99.315

0.384
  comP Acinetobacter baylyi ADP1

35.897

100

0.384
  pilA Haemophilus influenzae Rd KW20

36.486

100

0.37
  pilA Haemophilus influenzae 86-028NP

36.552

99.315

0.363