Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DVS74_RS10500 Genome accession   NZ_CP099516
Coordinates   2286742..2287188 (+) Length   148 a.a.
NCBI ID   WP_128283976.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain ESVL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2281742..2292188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVS74_RS10480 (DVS74_010470) - 2281990..2283327 (+) 1338 WP_027700147.1 sensor histidine kinase -
  DVS74_RS10485 (DVS74_010475) coaE 2283594..2284217 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  DVS74_RS10490 (DVS74_010480) pilD 2284229..2285092 (-) 864 WP_004084594.1 prepilin peptidase Machinery gene
  DVS74_RS10495 (DVS74_010485) pilC 2285099..2286301 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  DVS74_RS10500 (DVS74_010490) pilA 2286742..2287188 (+) 447 WP_128283976.1 pilin Machinery gene
  DVS74_RS10505 (DVS74_010495) - 2287433..2287660 (-) 228 WP_012338102.1 hypothetical protein -
  DVS74_RS10510 (DVS74_010500) pilA/pilAI 2288505..2288945 (+) 441 WP_012338104.1 pilin Machinery gene
  DVS74_RS10515 (DVS74_010505) pilB 2289785..2291518 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15402.58 Da        Isoelectric Point: 8.4988

>NTDB_id=602177 DVS74_RS10500 WP_128283976.1 2286742..2287188(+) (pilA) [Xylella fastidiosa subsp. multiplex strain ESVL]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKSTSNASDIGLRTDTTRCGI
TVKIDAAGTANITCKVKGNSQVNDKTIAWDRTPDNSAGTNGVNNGGVWTCSSNVTSDALRPSGCMAAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=602177 DVS74_RS10500 WP_128283976.1 2286742..2287188(+) (pilA) [Xylella fastidiosa subsp. multiplex strain ESVL]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGAGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAATCGATGCCGCTGGCACTGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCAACG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATGGCTGCTAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA Acinetobacter baumannii strain A118

40

100

0.405
  pilA Vibrio cholerae strain A1552

38.065

100

0.399
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.065

100

0.399
  pilA Vibrio cholerae C6706

38.065

100

0.399