Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K6749_RS13870 Genome accession   NZ_CP082310
Coordinates   2975171..2976916 (-) Length   581 a.a.
NCBI ID   WP_042523674.1    Uniprot ID   A0A7Y0R021
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2970171..2981916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13850 (K6749_13770) - 2970859..2971698 (-) 840 WP_238970115.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  K6749_RS13855 (K6749_13775) - 2971768..2973279 (-) 1512 WP_238966807.1 AAA family ATPase -
  K6749_RS13860 (K6749_13780) aroB 2973332..2974432 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K6749_RS13865 (K6749_13785) aroK 2974465..2974983 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6749_RS13870 (K6749_13790) pilQ 2975171..2976916 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6749_RS13875 (K6749_13795) pilP 2976942..2977457 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6749_RS13880 (K6749_13800) pilO 2977447..2978040 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6749_RS13885 (K6749_13805) pilN 2978033..2978608 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6749_RS13890 (K6749_13810) pilM 2978592..2979608 (-) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63648.66 Da        Isoelectric Point: 4.4326

>NTDB_id=601248 K6749_RS13870 WP_042523674.1 2975171..2976916(-) (pilQ) [Vibrio alginolyticus strain HYV1]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGPFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=601248 K6749_RS13870 WP_042523674.1 2975171..2976916(-) (pilQ) [Vibrio alginolyticus strain HYV1]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCCTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGTCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTTCCGCTATTAGGTGCGCTTTTTCGTCGA
AGTTATGAAAATGTTGGTAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0R021

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.415

100

0.394
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii D1279779

35.953

100

0.37
  pilQ Acinetobacter baumannii strain A118

35.953

100

0.37