Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K6749_RS13890 Genome accession   NZ_CP082310
Coordinates   2978592..2979608 (-) Length   338 a.a.
NCBI ID   WP_005382774.1    Uniprot ID   A0AAP5Z0M3
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973592..2984608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13865 (K6749_13785) aroK 2974465..2974983 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6749_RS13870 (K6749_13790) pilQ 2975171..2976916 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6749_RS13875 (K6749_13795) pilP 2976942..2977457 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6749_RS13880 (K6749_13800) pilO 2977447..2978040 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6749_RS13885 (K6749_13805) pilN 2978033..2978608 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6749_RS13890 (K6749_13810) pilM 2978592..2979608 (-) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene
  K6749_RS13895 (K6749_13815) - 2979753..2982314 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  K6749_RS13900 (K6749_13820) oxyR 2982367..2983278 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  K6749_RS13905 (K6749_13825) - 2983448..2984176 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=601252 K6749_RS13890 WP_005382774.1 2978592..2979608(-) (pilM) [Vibrio alginolyticus strain HYV1]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=601252 K6749_RS13890 WP_005382774.1 2978592..2979608(-) (pilM) [Vibrio alginolyticus strain HYV1]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACTATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503