Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K6749_RS13885 Genome accession   NZ_CP082310
Coordinates   2978033..2978608 (-) Length   191 a.a.
NCBI ID   WP_017821732.1    Uniprot ID   A0A2I3CBW9
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973033..2983608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13860 (K6749_13780) aroB 2973332..2974432 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K6749_RS13865 (K6749_13785) aroK 2974465..2974983 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6749_RS13870 (K6749_13790) pilQ 2975171..2976916 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6749_RS13875 (K6749_13795) pilP 2976942..2977457 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6749_RS13880 (K6749_13800) pilO 2977447..2978040 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6749_RS13885 (K6749_13805) pilN 2978033..2978608 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6749_RS13890 (K6749_13810) pilM 2978592..2979608 (-) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene
  K6749_RS13895 (K6749_13815) - 2979753..2982314 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  K6749_RS13900 (K6749_13820) oxyR 2982367..2983278 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22349.86 Da        Isoelectric Point: 7.4658

>NTDB_id=601251 K6749_RS13885 WP_017821732.1 2978033..2978608(-) (pilN) [Vibrio alginolyticus strain HYV1]
MLYRINLLPWRESQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=601251 K6749_RS13885 WP_017821732.1 2978033..2978608(-) (pilN) [Vibrio alginolyticus strain HYV1]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAGTCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3CBW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

63.277

92.67

0.586