Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   K6749_RS13880 Genome accession   NZ_CP082310
Coordinates   2977447..2978040 (-) Length   197 a.a.
NCBI ID   WP_042523672.1    Uniprot ID   A0A7Y0R197
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2972447..2983040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13860 (K6749_13780) aroB 2973332..2974432 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  K6749_RS13865 (K6749_13785) aroK 2974465..2974983 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K6749_RS13870 (K6749_13790) pilQ 2975171..2976916 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ family protein Machinery gene
  K6749_RS13875 (K6749_13795) pilP 2976942..2977457 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  K6749_RS13880 (K6749_13800) pilO 2977447..2978040 (-) 594 WP_042523672.1 type 4a pilus biogenesis protein PilO Machinery gene
  K6749_RS13885 (K6749_13805) pilN 2978033..2978608 (-) 576 WP_017821732.1 PilN domain-containing protein Machinery gene
  K6749_RS13890 (K6749_13810) pilM 2978592..2979608 (-) 1017 WP_005382774.1 type IV pilus assembly protein PilM Machinery gene
  K6749_RS13895 (K6749_13815) - 2979753..2982314 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22999.53 Da        Isoelectric Point: 5.0948

>NTDB_id=601250 K6749_RS13880 WP_042523672.1 2977447..2978040(-) (pilO) [Vibrio alginolyticus strain HYV1]
MVDWQDLEIDEMAEWPLLPQIVVIVLLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEAKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=601250 K6749_RS13880 WP_042523672.1 2977447..2978040(-) (pilO) [Vibrio alginolyticus strain HYV1]
GTGGTTGATTGGCAAGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGTACTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATTCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGTGCTAATACCTATCA
GTTTAAGCCGGAGGAGGCGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0R197

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685