Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6979_RS16375 Genome accession   NZ_CP082218
Coordinates   3874287..3875420 (+) Length   377 a.a.
NCBI ID   WP_104602238.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain IL_234     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3869287..3880420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6979_RS16350 (K6979_16290) - 3869459..3870658 (-) 1200 WP_104602242.1 cystathionine gamma-synthase -
  K6979_RS16355 (K6979_16295) - 3870749..3872119 (-) 1371 WP_104602369.1 pyridoxal-phosphate dependent enzyme -
  K6979_RS16360 (K6979_16300) - 3872357..3872575 (-) 219 WP_104602241.1 YdcH family protein -
  K6979_RS16365 (K6979_16305) - 3872918..3873808 (-) 891 WP_104602240.1 hypothetical protein -
  K6979_RS16370 (K6979_16310) - 3873805..3874188 (-) 384 WP_104602239.1 DUF4398 domain-containing protein -
  K6979_RS16375 (K6979_16315) pilU 3874287..3875420 (+) 1134 WP_104602238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6979_RS16380 (K6979_16320) maiA 3875690..3876352 (-) 663 WP_104602237.1 maleylacetoacetate isomerase -
  K6979_RS16385 (K6979_16325) - 3876387..3877376 (-) 990 WP_104602236.1 fumarylacetoacetate hydrolase family protein -
  K6979_RS16390 (K6979_16330) - 3877754..3879142 (+) 1389 WP_104602235.1 DEAD/DEAH box helicase -
  K6979_RS16395 (K6979_16335) queD 3879293..3879646 (+) 354 WP_104602234.1 6-carboxytetrahydropterin synthase QueD -
  K6979_RS16400 (K6979_16340) - 3879839..3880174 (+) 336 WP_104602233.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41633.94 Da        Isoelectric Point: 6.2909

>NTDB_id=600291 K6979_RS16375 WP_104602238.1 3874287..3875420(+) (pilU) [Xanthomonas cucurbitae strain IL_234]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIAELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKLSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGTDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=600291 K6979_RS16375 WP_104602238.1 3874287..3875420(+) (pilU) [Xanthomonas cucurbitae strain IL_234]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAGAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACCGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGATGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAACGCGGCGAGGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGC
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGGT
CGGGCAAGTCCACCTCGCTGGCTTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCTATCGAATACCTGCACAAGCACAAGCTGTCGATCGTGAATCAGCGCGAGGTGGGGCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACCATGGAGGCGG
CCATTGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCGCAGCG
CCTGGTCAAGGGCACGGATGGACGTCGCCGGCCGGCGACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGTCGGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAACACGACCCCTATGCCGACTACGAGGCGGCATCGGCCCCGAAGATCAGTC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366