Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6979_RS04695 Genome accession   NZ_CP082218
Coordinates   1146695..1147123 (+) Length   142 a.a.
NCBI ID   WP_104604285.1    Uniprot ID   A0A2S7DNL4
Organism   Xanthomonas cucurbitae strain IL_234     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1134245..1156066 1146695..1147123 within 0


Gene organization within MGE regions


Location: 1134245..1156066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6979_RS04640 (K6979_04630) - 1134245..1135345 (-) 1101 WP_159406553.1 IS5 family transposase -
  K6979_RS04645 (K6979_04635) - 1135719..1136111 (+) 393 WP_104604311.1 hypothetical protein -
  K6979_RS04650 (K6979_04640) - 1136199..1136588 (+) 390 WP_104604301.1 H-NS family nucleoid-associated regulatory protein -
  K6979_RS04655 (K6979_04645) glgX 1136716..1138848 (-) 2133 WP_104604299.1 glycogen debranching protein GlgX -
  K6979_RS04660 (K6979_04650) rimK 1139320..1140225 (+) 906 WP_104604297.1 30S ribosomal protein S6--L-glutamate ligase -
  K6979_RS04665 (K6979_04655) - 1140497..1140853 (+) 357 WP_146090337.1 hypothetical protein -
  K6979_RS04670 (K6979_04660) - 1140916..1141593 (+) 678 WP_003490678.1 response regulator transcription factor -
  K6979_RS04675 (K6979_04665) - 1142049..1143383 (+) 1335 WP_104604293.1 HAMP domain-containing sensor histidine kinase -
  K6979_RS04680 (K6979_04670) coaE 1143598..1144203 (-) 606 WP_104604291.1 dephospho-CoA kinase -
  K6979_RS04685 (K6979_04675) - 1144217..1145080 (-) 864 WP_104604289.1 A24 family peptidase -
  K6979_RS04690 (K6979_04680) pilC 1145087..1146349 (-) 1263 WP_104604287.1 type II secretion system F family protein Machinery gene
  K6979_RS04695 (K6979_04685) pilA 1146695..1147123 (+) 429 WP_104604285.1 pilin Machinery gene
  K6979_RS04700 (K6979_04690) pilB 1147261..1148994 (+) 1734 WP_104604283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6979_RS04705 (K6979_04695) pilR 1149631..1151022 (-) 1392 WP_170066996.1 sigma-54 dependent transcriptional regulator Regulator
  K6979_RS04710 (K6979_04700) - 1151182..1152795 (-) 1614 WP_104604280.1 HAMP domain-containing sensor histidine kinase -
  K6979_RS04715 (K6979_04705) sucC 1153030..1154199 (+) 1170 WP_104604278.1 ADP-forming succinate--CoA ligase subunit beta -
  K6979_RS04720 (K6979_04710) sucD 1154224..1155099 (+) 876 WP_104604276.1 succinate--CoA ligase subunit alpha -
  K6979_RS04725 (K6979_04715) - 1155217..1155453 (-) 237 WP_158251453.1 hypothetical protein -
  K6979_RS04730 (K6979_04720) - 1155674..1156066 (+) 393 WP_167526931.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14270.38 Da        Isoelectric Point: 9.1206

>NTDB_id=600278 K6979_RS04695 WP_104604285.1 1146695..1147123(+) (pilA) [Xanthomonas cucurbitae strain IL_234]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTVRGRVSEAMVAASAAKTVVAENAANGSALNSGWTAPTATTNVASVA
VAAATGNITVTTTAKAGDGTIIFAPTANGAALASGTVPTDRISWDCKGGTLAAKYRPAECRA

Nucleotide


Download         Length: 429 bp        

>NTDB_id=600278 K6979_RS04695 WP_104604285.1 1146695..1147123(+) (pilA) [Xanthomonas cucurbitae strain IL_234]
ATGAAGAAGCAACAAGGCTTTACTCTGATCGAACTGATGATCGTCGTCGCGATCATCGCTATCCTTGCCGCCATCGCACT
GCCGGCCTATCAGGACTACACCGTTCGCGGCCGTGTCTCTGAAGCGATGGTTGCCGCATCTGCCGCCAAGACGGTGGTTG
CCGAAAATGCCGCCAACGGCTCTGCCCTGAATAGCGGTTGGACTGCTCCTACGGCAACCACCAATGTTGCCAGCGTAGCC
GTTGCGGCCGCCACTGGTAACATCACTGTGACCACCACTGCCAAAGCCGGTGACGGTACGATCATTTTCGCTCCCACGGC
TAACGGCGCGGCACTGGCTTCCGGCACCGTTCCTACCGATCGTATTTCTTGGGATTGCAAGGGCGGTACCTTGGCTGCCA
AGTATCGTCCTGCTGAATGCCGCGCCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7DNL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.572

100

0.577
  pilA2 Legionella pneumophila str. Paris

54.795

100

0.563
  pilA2 Legionella pneumophila strain ERS1305867

54.795

100

0.563
  comP Acinetobacter baylyi ADP1

48.408

100

0.535
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.71

100

0.507
  pilA/pilA1 Eikenella corrodens VA1

39.355

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.096

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.426

99.296

0.401
  pilA Acinetobacter baumannii strain A118

39.716

99.296

0.394
  pilA Vibrio cholerae C6706

35.256

100

0.387
  pilA Vibrio cholerae strain A1552

35.256

100

0.387
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.256

100

0.387