Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K4A87_RS02880 Genome accession   NZ_CP082178
Coordinates   602915..604048 (-) Length   377 a.a.
NCBI ID   WP_208588939.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain SHQP01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597915..609048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4A87_RS02855 (K4A87_02840) - 598371..598733 (-) 363 WP_208588923.1 phasin family protein -
  K4A87_RS02860 (K4A87_02845) queD 598941..599294 (-) 354 WP_002814044.1 6-carboxytetrahydropterin synthase QueD -
  K4A87_RS02865 (K4A87_02850) - 599410..600807 (-) 1398 WP_208588927.1 DEAD/DEAH box helicase -
  K4A87_RS02870 (K4A87_02855) - 601185..602174 (+) 990 WP_208588929.1 fumarylacetoacetate hydrolase family protein -
  K4A87_RS02875 (K4A87_02860) maiA 602218..602889 (+) 672 WP_208588937.1 maleylacetoacetate isomerase -
  K4A87_RS02880 (K4A87_02865) pilU 602915..604048 (-) 1134 WP_208588939.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K4A87_RS02885 (K4A87_02870) - 604147..604530 (+) 384 WP_208588940.1 DUF4398 domain-containing protein -
  K4A87_RS02890 (K4A87_02875) - 604527..605417 (+) 891 WP_208588942.1 hypothetical protein -
  K4A87_RS02895 (K4A87_02880) - 605775..605993 (+) 219 WP_002814034.1 YdcH family protein -
  K4A87_RS02905 (K4A87_02890) - 606360..607730 (+) 1371 WP_208588944.1 pyridoxal-phosphate dependent enzyme -
  K4A87_RS02910 (K4A87_02895) - 607820..609016 (+) 1197 WP_208588946.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42106.36 Da        Isoelectric Point: 6.2928

>NTDB_id=599571 K4A87_RS02880 WP_208588939.1 602915..604048(-) (pilU) [Xanthomonas fragariae strain SHQP01]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGIVRKIAYSLMDEEQVRRFERELEFNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDIIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFQESAHKNVLMNLALNLRAVVSQRLVKGADGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRSADSRDGLALKFRLSEGSSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=599571 K4A87_RS02880 WP_208588939.1 602915..604048(-) (pilU) [Xanthomonas fragariae strain SHQP01]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCAGGAATAGTCAGGAAAATCGCATATTCGT
TGATGGACGAAGAGCAAGTGAGGCGGTTCGAGCGCGAGTTGGAATTCAACATGGCAATCGCCCTGCAGGATGCCGGGCGC
TTCCGCGTGAACGTGTTCAAACAGCGTGGCGAGGTGGGGATGGTGATCCGCGCCATCCGCAGCAGGATCCCCAGCATCGA
AGAACTCAATCTGCCGCAGGTGCTCAAGGACATCATCATGACACCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGTAAGTCGACCTCGCTGGCTTCGATGATCGACCACCGCAACAGCACTACTACCGGGCACATCCTCACTATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAATCAGCGCGAGGTAGGTCTGGATACGCATACCTTCCAAAG
CGCGCTAAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTAGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGTCTGGCGACGCTGCACTCCAACAATGCCGACCAGACTATCGAGCGCATC
CTCAATTTCTTCCAGGAAAGCGCGCACAAGAATGTGCTGATGAATCTGGCGCTGAATCTGCGCGCCGTCGTCTCACAGCG
GCTGGTCAAGGGCGCGGACGGGCGCCGCCGCCCCGCTACCGAAGTGCTGCTCAACACCCCGATGATTCGCGACCTACTGC
GTCGCGGCCAGGTGCACGAGGTCAAACAAGCGATGGAGGAATCTCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTCAAATCAGGCGAAATCGAGCAGGAGGAAGCGCTGCGCTCAGCCGACTCGCGCGATGGCCTGGCGTTGAA
GTTCCGCCTGTCCGAAGGCAGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAACGGCAGCGGCGCCGAAGATCAGTC
ACGGGTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(67-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.153

93.899

0.462
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.503

100

0.451
  pilT Pseudomonas aeruginosa PAK

42.012

89.655

0.377
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Legionella pneumophila strain Lp02

40.828

89.655

0.366
  pilT Legionella pneumophila strain ERS1305867

40.828

89.655

0.366