Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSYCG_RS01485 Genome accession   NC_021661
Coordinates   349205..350326 (-) Length   373 a.a.
NCBI ID   WP_020442647.1    Uniprot ID   -
Organism   Psychrobacter sp. G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344205..355326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYCG_RS01455 (PSYCG_01665) - 344755..345090 (+) 336 WP_020442643.1 c-type cytochrome -
  PSYCG_RS01460 (PSYCG_01670) - 345433..346101 (+) 669 WP_011512659.1 c-type cytochrome -
  PSYCG_RS01465 (PSYCG_01675) - 346358..347500 (+) 1143 WP_020442644.1 hypothetical protein -
  PSYCG_RS01470 (PSYCG_01680) - 347488..347892 (+) 405 WP_020442645.1 RnfH family protein -
  PSYCG_RS01475 (PSYCG_01685) - 347918..348334 (-) 417 WP_051130937.1 outer membrane protein assembly factor BamE -
  PSYCG_RS01480 (PSYCG_01690) fur 348659..349093 (+) 435 WP_011512663.1 ferric iron uptake transcriptional regulator -
  PSYCG_RS01485 (PSYCG_01695) pilU 349205..350326 (-) 1122 WP_020442647.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSYCG_RS01490 (PSYCG_01700) pilT 350485..351537 (-) 1053 WP_020442648.1 type IV pilus twitching motility protein PilT Machinery gene
  PSYCG_RS01495 (PSYCG_01705) - 351853..352611 (+) 759 WP_020442649.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSYCG_RS01500 (PSYCG_01710) - 352829..354103 (-) 1275 WP_020442650.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41674.86 Da        Isoelectric Point: 6.7809

>NTDB_id=59432 PSYCG_RS01485 WP_020442647.1 349205..350326(-) (pilU) [Psychrobacter sp. G]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTVNQQKEFDETNECQFAITDQAQQA
RFRVSAFIQRDMAGMILRKIENNIPTVEELRLPPSLKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGDVHEIKDVMTRSRDSGMQTFDQ
ALFDLYENGEITYKEAILHADSPNDLRLKIKLSSKNGVANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=59432 PSYCG_RS01485 WP_020442647.1 349205..350326(-) (pilU) [Psychrobacter sp. G]
ATGGACATTGATAAACTTTTACAATTGATGATTAACAAAAACGGCTCTGACCTTTTTATCACTGCTGATGTCGCCCCTTC
CATGAAAATCAATGGCAAAATACTGCCGGTTGGTAAAACGCCTTTAACTGCTGAGCAGACTATGAGGCTGGTTAAAGGCG
TCATGACAGTCAATCAGCAAAAAGAGTTTGATGAGACCAACGAGTGCCAGTTTGCGATTACTGATCAGGCGCAACAAGCA
CGTTTTAGAGTCAGTGCCTTTATACAACGTGATATGGCAGGGATGATTTTACGGAAAATCGAAAATAATATCCCTACTGT
CGAGGAGCTACGCCTGCCCCCCTCTCTAAAAGAGCTGGCAATGAAAAAGCGTGGTATAATTTTATTAGTAGGTGCAACCG
GTACGGGTAAATCAACCACCCTTGCGGCAATGATAGGACATCGCAATCAAAACTCGCATGGTCATATTATTACCATAGAA
GATCCTATCGAGTTTGTCCATAAGCATCGTGGCTGTATTATTACCCAGCGCGAAGTCGGTATCGATACCCACTCTTTTGA
AGCCGGTCTAAAAAACACCTTGCGCCAAGCGCCAGATGTGATTTTGATCGGTGAGATTCGCAATCGCGAAGTCATGAGTT
ATGCGATTCAATATGCTGAAACGGGGCATTTGGTATTTGCAACCTTGCACGCCAATAATGCCAACCAAGCAATTGATCGT
ATTATTCACTTTTTTGAGACCAGTCGCCATAATCAATTGTTCATGGATTTATCACTTAACTTACAAGCCATTATCGCGCA
GCAGTTGATTCCAACGCCTGACGGTAAGGGACGCCGCGCTGCTATTGAAATTTTATTAAACTCACAGCTGATTAGCGATT
ATATTCGTAAAGGCGATGTGCATGAAATCAAAGATGTCATGACACGCTCACGTGATAGCGGTATGCAGACATTTGACCAA
GCACTGTTTGATCTATATGAAAACGGTGAGATTACTTATAAAGAAGCGATTCTTCATGCAGACTCTCCCAATGATTTGCG
CTTAAAAATCAAACTCAGCAGCAAAAATGGCGTGGCAAACTTGGATGAAGGCGCAGACAGTTTATCGTTAGATATTACTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.952

100

0.66
  pilU Acinetobacter baylyi ADP1

66.479

95.174

0.633
  pilU Vibrio cholerae strain A1552

51.902

98.66

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.803

98.123

0.41
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter baumannii strain A118

39.65

91.957

0.365
  pilT Acinetobacter nosocomialis M2

39.65

91.957

0.365
  pilT Acinetobacter baumannii D1279779

39.65

91.957

0.365
  pilT Legionella pneumophila strain ERS1305867

41.538

87.131

0.362
  pilT Legionella pneumophila strain Lp02

41.538

87.131

0.362

Multiple sequence alignment