Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0J45_RS05705 Genome accession   NZ_CP080422
Coordinates   1282894..1284006 (+) Length   370 a.a.
NCBI ID   WP_220044603.1    Uniprot ID   -
Organism   Shewanella alkalitolerans strain FJAT-54031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1277894..1289006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0J45_RS05670 (K0J45_05670) rdgB 1278042..1278641 (-) 600 WP_220044599.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0J45_RS05675 (K0J45_05675) - 1278783..1279217 (-) 435 WP_011864937.1 DUF4426 domain-containing protein -
  K0J45_RS05680 (K0J45_05680) yggU 1279304..1279594 (-) 291 WP_220044600.1 DUF167 family protein YggU -
  K0J45_RS05685 (K0J45_05685) - 1279595..1280143 (-) 549 WP_144201230.1 YggT family protein -
  K0J45_RS05690 (K0J45_05690) proC 1280204..1281022 (-) 819 WP_220044601.1 pyrroline-5-carboxylate reductase -
  K0J45_RS05695 (K0J45_05695) - 1281107..1281808 (-) 702 WP_220044602.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0J45_RS05700 (K0J45_05700) pilT 1281844..1282881 (+) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  K0J45_RS05705 (K0J45_05705) pilU 1282894..1284006 (+) 1113 WP_220044603.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0J45_RS05710 (K0J45_05710) hemH 1284186..1285199 (+) 1014 WP_220044604.1 ferrochelatase -
  K0J45_RS05715 (K0J45_05715) - 1286000..1286344 (+) 345 WP_220044605.1 hypothetical protein -
  K0J45_RS05720 (K0J45_05720) - 1286461..1286817 (+) 357 WP_220044606.1 DUF6559 family protein -
  K0J45_RS05725 (K0J45_05725) ruvX 1287254..1287679 (-) 426 WP_220044607.1 Holliday junction resolvase RuvX -
  K0J45_RS05730 (K0J45_05730) - 1287701..1288258 (-) 558 WP_220044608.1 YqgE/AlgH family protein -
  K0J45_RS05735 (K0J45_05735) yciH 1288275..1288604 (-) 330 WP_011864948.1 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41129.24 Da        Isoelectric Point: 6.3733

>NTDB_id=592847 K0J45_RS05705 WP_220044603.1 1282894..1284006(+) (pilU) [Shewanella alkalitolerans strain FJAT-54031]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKGLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGSGRRAAIEVLINTPRVASIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592847 K0J45_RS05705 WP_220044603.1 1282894..1284006(+) (pilU) [Shewanella alkalitolerans strain FJAT-54031]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTAGAGCGCAAGGCGTCGGATCTCTTCATCACCGCCGGCTTTCCCCCCAG
CGCTAAGGTTGATGGCGAGGTGCGCCCCTTAGCCGAGAACCCTTTTACCCCGGCTCAGTCGCTGGAGTTTGTCGAGGCCC
TGATGACAGAGGCCCAGCGCAAAGAGTTTCATGAGACCCGCGAATGTAACTTCGCCTATGGCGAGAAGGGCCTGGGACGT
TTTCGTGTCAGCGCCTTCTGGCAGCGCGAGTCGCCGGGCTGCGTGATGCGTCGCATCGAGACCAAGATCCCGCTGGTGGA
AGATCTAAAGCTGCCCCCAATTCTGAAAGACCTGGTGATGAGCAAGCGTGGCCTTATCATCATGGTGGGTGGTACAGGTA
CAGGTAAGTCGACCTCGTTAGCGGCACTGGTGGGTTACCGCAACTCCCACGCCCGCGGCCATATCCTCACCATCGAAGAT
CCGGTGGAATTTGTCCACGACCATCGCAAGAGCATCATCACCCAGCGTGAGGTGGGCATAGATACCGACTCTTTCGATGC
CGCGCTCAAGAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGTACTCAGGAGACCATGGAGTTTG
CCCTGTCGTTCGCCGAGACAGGTCACCTCTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCCGAGAGTAAGCACCAGCAGCTGCTGTTCGACTTGTCGCTGAACCTTCGCGGCATCGTGGCGCAGCA
ACTTGTGCCCAAGGCCGATGGCTCGGGTCGCCGTGCGGCCATCGAGGTGTTGATCAACACCCCAAGGGTTGCCAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCCAAATCCAACGAGCAGGGGATGCAGACCTTCGACCAGGCG
CTGCTCAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCGCTGCACCACGCCGACTCGCCAAACGATCTGCGCTT
GATGATTAAGCTGCAAAGCTCAGAGTCTGCTAGCTCAGGCTTCATGGAAGGTGTGACGCTGGATCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Acinetobacter baylyi ADP1

59.889

97.027

0.581
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362