Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0I63_RS05775 Genome accession   NZ_CP080420
Coordinates   1293488..1294600 (+) Length   370 a.a.
NCBI ID   WP_011864943.1    Uniprot ID   A3QC12
Organism   Shewanella rhizosphaerae strain FJAT-53764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1288488..1299600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I63_RS05740 (K0I63_05740) rdgB 1288639..1289238 (-) 600 WP_220058771.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0I63_RS05745 (K0I63_05745) - 1289380..1289814 (-) 435 WP_011864937.1 DUF4426 domain-containing protein -
  K0I63_RS05750 (K0I63_05750) yggU 1289901..1290191 (-) 291 WP_220082827.1 DUF167 family protein YggU -
  K0I63_RS05755 (K0I63_05755) - 1290192..1290740 (-) 549 WP_220082828.1 YggT family protein -
  K0I63_RS05760 (K0I63_05760) proC 1290801..1291619 (-) 819 WP_220082829.1 pyrroline-5-carboxylate reductase -
  K0I63_RS05765 (K0I63_05765) - 1291701..1292402 (-) 702 WP_011864941.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0I63_RS05770 (K0I63_05770) pilT 1292438..1293475 (+) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  K0I63_RS05775 (K0I63_05775) pilU 1293488..1294600 (+) 1113 WP_011864943.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0I63_RS05780 (K0I63_05780) hemH 1294781..1295794 (+) 1014 WP_220082830.1 ferrochelatase -
  K0I63_RS05785 (K0I63_05785) - 1296539..1296994 (+) 456 WP_220082831.1 hypothetical protein -
  K0I63_RS05790 (K0I63_05790) - 1297001..1297537 (+) 537 WP_220082832.1 hypothetical protein -
  K0I63_RS05795 (K0I63_05795) - 1297694..1298329 (+) 636 WP_220082833.1 CPBP family intramembrane glutamic endopeptidase -
  K0I63_RS05800 (K0I63_05800) - 1298345..1298692 (+) 348 WP_220082834.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41143.26 Da        Isoelectric Point: 6.3733

>NTDB_id=592802 K0I63_RS05775 WP_011864943.1 1293488..1294600(+) (pilU) [Shewanella rhizosphaerae strain FJAT-53764]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKGLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEVLINTPRVASIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592802 K0I63_RS05775 WP_011864943.1 1293488..1294600(+) (pilU) [Shewanella rhizosphaerae strain FJAT-53764]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTAGAGCGCAAGGCGTCGGATCTCTTCATCACCGCCGGCTTTCCCCCCAG
CGCTAAGGTTGATGGCGAGGTGCGCCCCTTAGCCGAGAACCCTTTTACTCCGGCTCAGTCGCTGGAGTTTGTTGAGGCCT
TGATGACAGAGGCCCAGCGTAAAGAGTTTCACGAGACCCGCGAGTGTAACTTCGCCTATGGCGAGAAGGGCCTGGGACGT
TTTCGTGTCAGCGCCTTCTGGCAGCGCGAGTCGCCGGGCTGCGTGATGCGTCGCATCGAGACCAAGATCCCGCTGGTGGA
AGATCTTAAGCTGCCACCAATCCTGAAAGACCTGGTGATGAGTAAGCGTGGCCTTATCATCATGGTGGGTGGTACGGGTA
CAGGTAAGTCGACCTCGCTGGCGGCGCTGGTGGGTTACCGCAACTCCCACGCCCGCGGCCATATCCTCACCATCGAAGAT
CCGGTGGAATTTGTCCACGACCATCGTAAGAGCATCATCACCCAGCGTGAGGTGGGCATAGATACCGACTCTTTCGATGC
CGCGCTCAAGAGTTCACTGCGTCAGGCGCCGGATGTGATCCTGATCGGTGAGATCCGTACCCAGGAGACCATGGAGTTTG
CTCTGTCGTTCGCCGAGACGGGTCACCTCTGTATGGCTACATTGCACGCCAACAACGCTAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCCGAGAGTAAGCACCAGCAGCTGCTGTTCGACTTGTCGCTGAACCTTCGCGGCATCGTGGCGCAGCA
ACTTGTGCCTAAGGCAGATGGCACGGGTCGCCGGGCGGCCATCGAGGTGTTGATCAACACCCCAAGGGTTGCCAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCCAAATCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
CTGCTTAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCGCTGCACCACGCCGACTCGCCAAACGATCTGCGCTT
GATGATCAAGCTGCAAAGCTCAGAATCGGCAAGCTCAGGTTTCATGGAAGGTGTGACGCTGGATCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3QC12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Acinetobacter baylyi ADP1

59.889

97.027

0.581
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362