Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   CLOPA_RS01590 Genome accession   NC_021182
Coordinates   336917..337318 (+) Length   133 a.a.
NCBI ID   WP_015613718.1    Uniprot ID   R4K0U4
Organism   Clostridium pasteurianum BC1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 305990..364639 336917..337318 within 0


Gene organization within MGE regions


Location: 305990..364639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOPA_RS01430 (Clopa_0294) - 305990..306706 (+) 717 WP_015613684.1 ABC transporter ATP-binding protein -
  CLOPA_RS01435 (Clopa_0295) - 306703..307899 (+) 1197 WP_015613685.1 ABC transporter permease -
  CLOPA_RS01440 (Clopa_0296) - 307928..308896 (+) 969 Protein_253 glycosyltransferase family 2 protein -
  CLOPA_RS01445 (Clopa_0297) - 309134..309817 (+) 684 WP_015613686.1 hypothetical protein -
  CLOPA_RS01450 (Clopa_0298) nadA 310112..311023 (+) 912 WP_015613687.1 quinolinate synthase NadA -
  CLOPA_RS25740 - 311025..312328 (+) 1304 Protein_256 L-aspartate oxidase -
  CLOPA_RS25745 nadC 312309..313145 (+) 837 WP_207637894.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CLOPA_RS01460 (Clopa_0300) - 314013..315392 (+) 1380 WP_015613689.1 radical SAM protein -
  CLOPA_RS01465 (Clopa_0301) - 316041..317318 (+) 1278 WP_015613690.1 ABC transporter substrate-binding protein -
  CLOPA_RS01470 (Clopa_0302) - 317586..318731 (-) 1146 WP_015613691.1 glycosyltransferase family 1 protein -
  CLOPA_RS01475 (Clopa_0303) - 319012..320028 (+) 1017 WP_041711109.1 CotS family spore coat protein -
  CLOPA_RS01480 (Clopa_0304) - 320025..320237 (+) 213 WP_015613693.1 hypothetical protein -
  CLOPA_RS01485 (Clopa_0305) - 320244..321002 (+) 759 WP_015613694.1 hypothetical protein -
  CLOPA_RS01490 (Clopa_0306) - 321027..322055 (+) 1029 WP_015613695.1 CotS family spore coat protein -
  CLOPA_RS01495 (Clopa_0307) - 322133..323260 (-) 1128 WP_015613696.1 glycosyltransferase family 1 protein -
  CLOPA_RS01500 (Clopa_0308) - 323543..324547 (+) 1005 WP_015613697.1 CotS family spore coat protein -
  CLOPA_RS01505 (Clopa_0309) - 324664..325443 (+) 780 WP_015613698.1 sporulation peptidase YabG -
  CLOPA_RS01510 (Clopa_0310) - 325485..327143 (-) 1659 WP_015613699.1 recombinase family protein -
  CLOPA_RS01515 (Clopa_0311) - 327247..328002 (-) 756 WP_015613700.1 hypothetical protein -
  CLOPA_RS25750 (Clopa_0312) - 328090..329583 (-) 1494 WP_015613701.1 GIY-YIG nuclease family protein -
  CLOPA_RS01525 (Clopa_0313) - 329680..330015 (-) 336 WP_015613702.1 hypothetical protein -
  CLOPA_RS01530 (Clopa_0314) - 330047..330466 (-) 420 WP_015613703.1 helix-turn-helix transcriptional regulator -
  CLOPA_RS01535 (Clopa_0315) - 330642..330848 (+) 207 WP_015613704.1 helix-turn-helix transcriptional regulator -
  CLOPA_RS24675 (Clopa_0316) - 330988..331143 (+) 156 WP_015613705.1 hypothetical protein -
  CLOPA_RS24680 - 331287..331424 (+) 138 WP_155241850.1 hypothetical protein -
  CLOPA_RS01545 (Clopa_0318) - 331421..331957 (+) 537 WP_015613707.1 helix-turn-helix transcriptional regulator -
  CLOPA_RS25485 (Clopa_0319) - 331963..332133 (+) 171 WP_172638596.1 hypothetical protein -
  CLOPA_RS01550 (Clopa_0320) - 332176..332481 (+) 306 WP_155241851.1 hypothetical protein -
  CLOPA_RS23500 (Clopa_0321) - 332578..333141 (+) 564 WP_015613710.1 hypothetical protein -
  CLOPA_RS23505 (Clopa_0322) - 333141..334262 (+) 1122 WP_015613711.1 hypothetical protein -
  CLOPA_RS01565 (Clopa_0323) - 334290..334592 (+) 303 WP_015613712.1 hypothetical protein -
  CLOPA_RS24065 (Clopa_0324) - 334597..335058 (+) 462 WP_015613713.1 hypothetical protein -
  CLOPA_RS01575 (Clopa_0325) - 335312..336010 (+) 699 WP_015613714.1 amino acid racemase -
  CLOPA_RS01580 (Clopa_0326) - 336159..336344 (+) 186 WP_015613715.1 hypothetical protein -
  CLOPA_RS01585 (Clopa_0327) - 336375..336698 (+) 324 WP_015613716.1 hypothetical protein -
  CLOPA_RS24685 (Clopa_0328) - 336763..336906 (+) 144 WP_015613717.1 hypothetical protein -
  CLOPA_RS01590 (Clopa_0329) ssb 336917..337318 (+) 402 WP_015613718.1 single-stranded DNA-binding protein Machinery gene
  CLOPA_RS01595 (Clopa_0330) - 337455..337796 (+) 342 WP_015613719.1 DNA-directed RNA polymerase sigma-70 factor -
  CLOPA_RS01600 (Clopa_0331) - 338013..338357 (+) 345 WP_015613720.1 hypothetical protein -
  CLOPA_RS25490 (Clopa_0332) - 338428..338595 (+) 168 WP_015613721.1 hypothetical protein -
  CLOPA_RS01605 (Clopa_0333) - 338595..339245 (+) 651 WP_015613722.1 tyrosine-type recombinase/integrase -
  CLOPA_RS01610 (Clopa_0334) - 339510..339983 (+) 474 WP_015613723.1 hypothetical protein -
  CLOPA_RS01615 (Clopa_0335) - 339985..340311 (+) 327 WP_015613724.1 HNH endonuclease -
  CLOPA_RS01620 (Clopa_0336) - 340435..340743 (+) 309 WP_015613725.1 P27 family phage terminase small subunit -
  CLOPA_RS01625 (Clopa_0337) - 340730..342388 (+) 1659 WP_015613726.1 terminase TerL endonuclease subunit -
  CLOPA_RS24690 (Clopa_0338) - 342403..342558 (+) 156 WP_015613727.1 hypothetical protein -
  CLOPA_RS01630 (Clopa_0339) - 342595..343770 (+) 1176 WP_015613728.1 phage portal protein -
  CLOPA_RS01635 (Clopa_0340) - 343745..344485 (+) 741 WP_015613729.1 head maturation protease, ClpP-related -
  CLOPA_RS01640 (Clopa_0341) - 344487..345938 (+) 1452 WP_015613730.1 phage major capsid protein -
  CLOPA_RS01645 (Clopa_0342) - 345951..346265 (+) 315 WP_015613731.1 hypothetical protein -
  CLOPA_RS01650 (Clopa_0343) - 346262..346606 (+) 345 WP_015613732.1 hypothetical protein -
  CLOPA_RS01655 (Clopa_0344) - 346603..346998 (+) 396 WP_015613733.1 hypothetical protein -
  CLOPA_RS01660 (Clopa_0345) - 346985..347299 (+) 315 WP_015613734.1 hypothetical protein -
  CLOPA_RS01665 (Clopa_0346) - 347312..347857 (+) 546 WP_015613735.1 hypothetical protein -
  CLOPA_RS01670 (Clopa_0347) - 347881..348180 (+) 300 WP_015613736.1 hypothetical protein -
  CLOPA_RS23510 (Clopa_0349) - 348484..351639 (+) 3156 WP_015613738.1 phage tail tape measure protein -
  CLOPA_RS01680 (Clopa_0350) - 351636..352337 (+) 702 WP_015613739.1 phage tail domain-containing protein -
  CLOPA_RS01685 (Clopa_0351) - 352414..353895 (+) 1482 WP_015613740.1 hypothetical protein -
  CLOPA_RS01690 (Clopa_0352) - 353897..356239 (+) 2343 WP_015613741.1 cellulase N-terminal Ig-like domain-containing protein -
  CLOPA_RS01695 (Clopa_0353) - 356339..357394 (+) 1056 WP_242834252.1 acyltransferase -
  CLOPA_RS01705 (Clopa_0354) - 357619..358839 (+) 1221 WP_015613743.1 family 16 glycosylhydrolase -
  CLOPA_RS01710 (Clopa_0355) - 359110..361077 (+) 1968 WP_015613744.1 phage tail protein -
  CLOPA_RS01715 (Clopa_0356) - 361127..361441 (+) 315 WP_015613745.1 hypothetical protein -
  CLOPA_RS01720 (Clopa_0357) - 361447..362406 (+) 960 WP_015613746.1 GH25 family lysozyme -
  CLOPA_RS01725 (Clopa_0358) - 362466..362837 (+) 372 WP_015613747.1 hypothetical protein -
  CLOPA_RS01730 (Clopa_0359) - 363119..364639 (+) 1521 WP_015613748.1 FG-GAP-like repeat-containing protein -

Sequence


Protein


Download         Length: 133 a.a.        Molecular weight: 14636.54 Da        Isoelectric Point: 5.9247

>NTDB_id=58545 CLOPA_RS01590 WP_015613718.1 336917..337318(+) (ssb) [Clostridium pasteurianum BC1]
MNRVVLIGRLTKDPELKFTPGTGYAVATFAIAVDRRVPNKEGVREADFIPIVIWGKQAESTANYMSKGKLVGISGRIQTR
SYDAKDGSKKYITEVVADEIQFLEWGKKNEGSNHNAEYEAAGITPVDDGDVPF

Nucleotide


Download         Length: 402 bp        

>NTDB_id=58545 CLOPA_RS01590 WP_015613718.1 336917..337318(+) (ssb) [Clostridium pasteurianum BC1]
ATGAATAGGGTAGTTTTGATAGGAAGATTAACAAAAGATCCAGAGCTTAAGTTTACTCCTGGTACTGGGTATGCAGTTGC
AACATTTGCTATAGCAGTGGATAGACGAGTACCTAATAAAGAAGGAGTAAGGGAAGCTGATTTTATTCCTATAGTAATAT
GGGGAAAGCAAGCTGAATCCACAGCTAATTATATGAGTAAAGGCAAGCTTGTAGGCATTAGTGGAAGAATACAAACTAGA
TCCTATGATGCTAAAGATGGCAGTAAGAAATATATAACTGAAGTAGTTGCTGATGAAATACAATTTCTTGAATGGGGAAA
AAAGAATGAAGGTAGTAATCATAATGCTGAATATGAAGCAGCTGGAATAACTCCTGTAGATGATGGGGACGTGCCATTTT
AG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB R4K0U4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

44.444

100

0.481

  ssbA Bacillus subtilis subsp. subtilis str. 168

56.731

78.195

0.444

  ssbB/cilA Streptococcus mitis NCTC 12261

40.152

99.248

0.399

  ssbB Bacillus subtilis subsp. subtilis str. 168

50.962

78.195

0.398

  ssbB Streptococcus sobrinus strain NIDR 6715-7

39.394

99.248

0.391

  ssbB/cilA Streptococcus pneumoniae TIGR4

39.394

99.248

0.391

  ssbB/cilA Streptococcus mitis SK321

38.636

99.248

0.383

  ssbB/cilA Streptococcus pneumoniae Rx1

38.636

99.248

0.383

  ssbB/cilA Streptococcus pneumoniae D39

38.636

99.248

0.383

  ssbB/cilA Streptococcus pneumoniae R6

38.636

99.248

0.383

  ssbB Lactococcus lactis subsp. cremoris KW2

46.667

78.947

0.368

  ssbA Streptococcus mutans UA159

37.121

99.248

0.368

  ssb Neisseria gonorrhoeae MS11

36.641

98.496

0.361


Multiple sequence alignment