Detailed information NTG0580933

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: I6L30_RS12485
• Genome accession: NZ_CP077365
• Coordinates: 2643529..2644587 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain FDAARGOS 1400
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2638529..2649587

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L30_RS12470 (I6L30_12470)	-	2639636..2639968 (+)	333	WP_077163639.1	hypothetical protein	-
I6L30_RS12475 (I6L30_12475)	-	2639971..2640795 (+)	825	WP_216984452.1	putative RNA methyltransferase	-
I6L30_RS12480 (I6L30_12480)	ponA	2640812..2643367 (-)	2556	WP_216984453.1	penicillin-binding protein PBP1a	-
I6L30_RS12485 (I6L30_12485)	pilM	2643529..2644587 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
I6L30_RS12490 (I6L30_12490)	pilN	2644587..2645231 (+)	645	WP_077163636.1	PilN domain-containing protein	Machinery gene
I6L30_RS12495 (I6L30_12495)	pilO	2645228..2645968 (+)	741	WP_216984454.1	type 4a pilus biogenesis protein PilO	Machinery gene
I6L30_RS12500 (I6L30_12500)	pilP	2645979..2646506 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
I6L30_RS12505 (I6L30_12505)	pilQ	2646568..2648694 (+)	2127	WP_049065605.1	type IV pilus secretin PilQ family protein	Machinery gene
I6L30_RS12510 (I6L30_12510)	aroK	2648706..2649275 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=580933 I6L30_RS12485 WP_004705187.1 2643529..2644587(+) (pilM) [Acinetobacter seifertii strain FDAARGOS 1400]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=580933 I6L30_RS12485 WP_004705187.1 2643529..2644587(+) (pilM) [Acinetobacter seifertii strain FDAARGOS 1400]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAAGCAGTAGCAGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACCAAAAATGCTGCAATT
GCTGTTCCTACATCGACAGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCTGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGGGCGAAAAAAGATCGTTCTTTACCAGATGACTATGAAATTGAAGTACTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTACTTGCAGGCG
GAAATGCGAATATTCCAGGTCTCGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426