Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   I6L30_RS12495 Genome accession   NZ_CP077365
Coordinates   2645228..2645968 (+) Length   246 a.a.
NCBI ID   WP_216984454.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain FDAARGOS 1400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640228..2650968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L30_RS12480 (I6L30_12480) ponA 2640812..2643367 (-) 2556 WP_216984453.1 penicillin-binding protein PBP1a -
  I6L30_RS12485 (I6L30_12485) pilM 2643529..2644587 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  I6L30_RS12490 (I6L30_12490) pilN 2644587..2645231 (+) 645 WP_077163636.1 PilN domain-containing protein Machinery gene
  I6L30_RS12495 (I6L30_12495) pilO 2645228..2645968 (+) 741 WP_216984454.1 type 4a pilus biogenesis protein PilO Machinery gene
  I6L30_RS12500 (I6L30_12500) pilP 2645979..2646506 (+) 528 WP_004705193.1 pilus assembly protein PilP Machinery gene
  I6L30_RS12505 (I6L30_12505) pilQ 2646568..2648694 (+) 2127 WP_049065605.1 type IV pilus secretin PilQ family protein Machinery gene
  I6L30_RS12510 (I6L30_12510) aroK 2648706..2649275 (+) 570 WP_004705195.1 shikimate kinase AroK -
  I6L30_RS12515 (I6L30_12515) aroB 2649304..2650386 (+) 1083 WP_216984455.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27848.54 Da        Isoelectric Point: 5.1340

>NTDB_id=580935 I6L30_RS12495 WP_216984454.1 2645228..2645968(+) (pilO) [Acinetobacter seifertii strain FDAARGOS 1400]
MSQDELHELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDSKSDIPVLNYSIKAKTYRYVEATDTAKQSTQSPTTPASTS
TTAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=580935 I6L30_RS12495 WP_216984454.1 2645228..2645968(+) (pilO) [Acinetobacter seifertii strain FDAARGOS 1400]
ATGAGTCAAGATGAACTTCACGAGTTATCATTAGAGCAATCGGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCCTTGGGTTATTTTATTGCCATTCAGCCAAAGCTACAAGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCAGCAGTATCAAGTTCAACTTCAGGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTAGTTGAAGATATCAATTTAA
CGGGTGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAGAATGAAGTAAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCTTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATTGTAAC
CATGCACGACTTTACTGTTGATGTATCTCCAGCGAAAGACAGCAAGTCAGATATTCCTGTATTGAACTATTCTATCAAAG
CGAAAACCTATCGCTATGTAGAAGCAACAGATACAGCGAAGCAATCGACCCAATCTCCAACAACACCTGCTTCAACTAGC
ACGACTGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

91.87

100

0.919
  comO Acinetobacter baylyi ADP1

68.571

99.593

0.683