Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SPN032672_RS05530 Genome accession   NC_021003
Coordinates   1045083..1046024 (+) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae SPN032672     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040083..1051024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN032672_RS05510 - 1040149..1041966 (+) 1818 WP_001220841.1 acyltransferase family protein -
  SPN032672_RS05515 nagA 1042119..1043270 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  SPN032672_RS05520 - 1043433..1044491 (+) 1059 WP_041178985.1 zinc-dependent alcohol dehydrogenase family protein -
  SPN032672_RS05525 - 1044642..1045007 (+) 366 WP_000286415.1 DUF1033 family protein -
  SPN032672_RS05530 comGA/cglA/cilD 1045083..1046024 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  SPN032672_RS05535 comGB/cglB 1045972..1046988 (+) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPN032672_RS05540 comGC/cglC 1046990..1047316 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  SPN032672_RS05545 comGD/cglD 1047309..1047713 (+) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPN032672_RS05550 comGE/cglE 1047676..1047978 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPN032672_RS05555 comGF/cglF 1047941..1048402 (+) 462 WP_000250537.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPN032672_RS05560 comGG/cglG 1048380..1048772 (+) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPN032672_RS05565 - 1049446..1049922 (-) 477 WP_000777686.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=57871 SPN032672_RS05530 WP_000249571.1 1045083..1046024(+) (comGA/cglA/cilD) [Streptococcus pneumoniae SPN032672]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=57871 SPN032672_RS05530 WP_000249571.1 1045083..1046024(+) (comGA/cglA/cilD) [Streptococcus pneumoniae SPN032672]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAAC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment