Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SPN032672_RS05555 Genome accession   NC_021003
Coordinates   1047941..1048402 (+) Length   153 a.a.
NCBI ID   WP_000250537.1    Uniprot ID   -
Organism   Streptococcus pneumoniae SPN032672     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1042941..1053402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN032672_RS05520 - 1043433..1044491 (+) 1059 WP_041178985.1 zinc-dependent alcohol dehydrogenase family protein -
  SPN032672_RS05525 - 1044642..1045007 (+) 366 WP_000286415.1 DUF1033 family protein -
  SPN032672_RS05530 comGA/cglA/cilD 1045083..1046024 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  SPN032672_RS05535 comGB/cglB 1045972..1046988 (+) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPN032672_RS05540 comGC/cglC 1046990..1047316 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  SPN032672_RS05545 comGD/cglD 1047309..1047713 (+) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPN032672_RS05550 comGE/cglE 1047676..1047978 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPN032672_RS05555 comGF/cglF 1047941..1048402 (+) 462 WP_000250537.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPN032672_RS05560 comGG/cglG 1048380..1048772 (+) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPN032672_RS05565 - 1049446..1049922 (-) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  SPN032672_RS05580 comYH 1051220..1052173 (+) 954 WP_000345118.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=57876 SPN032672_RS05555 WP_000250537.1 1047941..1048402(+) (comGF/cglF) [Streptococcus pneumoniae SPN032672]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGN
RLYMKQDDKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=57876 SPN032672_RS05555 WP_000250537.1 1047941..1048402(+) (comGF/cglF) [Streptococcus pneumoniae SPN032672]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGGCCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGACAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis SK321

92.157

100

0.922
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909

Multiple sequence alignment