Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SPN032672_RS05535 Genome accession   NC_021003
Coordinates   1045972..1046988 (+) Length   338 a.a.
NCBI ID   WP_001844019.1    Uniprot ID   -
Organism   Streptococcus pneumoniae SPN032672     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040972..1051988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN032672_RS05515 nagA 1042119..1043270 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  SPN032672_RS05520 - 1043433..1044491 (+) 1059 WP_041178985.1 zinc-dependent alcohol dehydrogenase family protein -
  SPN032672_RS05525 - 1044642..1045007 (+) 366 WP_000286415.1 DUF1033 family protein -
  SPN032672_RS05530 comGA/cglA/cilD 1045083..1046024 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  SPN032672_RS05535 comGB/cglB 1045972..1046988 (+) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPN032672_RS05540 comGC/cglC 1046990..1047316 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  SPN032672_RS05545 comGD/cglD 1047309..1047713 (+) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPN032672_RS05550 comGE/cglE 1047676..1047978 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPN032672_RS05555 comGF/cglF 1047941..1048402 (+) 462 WP_000250537.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPN032672_RS05560 comGG/cglG 1048380..1048772 (+) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPN032672_RS05565 - 1049446..1049922 (-) 477 WP_000777686.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38540.51 Da        Isoelectric Point: 9.4800

>NTDB_id=57872 SPN032672_RS05535 WP_001844019.1 1045972..1046988(+) (comGB/cglB) [Streptococcus pneumoniae SPN032672]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=57872 SPN032672_RS05535 WP_001844019.1 1045972..1046988(+) (comGB/cglB) [Streptococcus pneumoniae SPN032672]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGATTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.238

99.408

0.698
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment