Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   KFS84_RS15420 Genome accession   NZ_CP076254
Coordinates   3455037..3455447 (-) Length   136 a.a.
NCBI ID   WP_039955568.1    Uniprot ID   A0A1M4L1M0
Organism   Xanthomonas translucens pv. graminis strain LMG 726     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3449491..3470766 3455037..3455447 within 0
IS/Tn 3454022..3454978 3455037..3455447 flank 59


Gene organization within MGE regions


Location: 3449491..3470766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFS84_RS15375 (KFS84_15315) - 3449491..3449787 (+) 297 WP_009580424.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KFS84_RS15380 (KFS84_15320) - 3449790..3450083 (+) 294 WP_009580422.1 addiction module antidote protein -
  KFS84_RS15385 (KFS84_15325) pilB 3450111..3451847 (-) 1737 WP_009580420.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFS84_RS15390 (KFS84_15330) - 3452054..3452344 (+) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KFS84_RS15395 (KFS84_15335) - 3452341..3452661 (+) 321 WP_009580416.1 DUF86 domain-containing protein -
  KFS84_RS15400 - 3452761..3453012 (+) 252 WP_009580414.1 transposase -
  KFS84_RS15405 - 3453013..3453318 (+) 306 WP_231103532.1 hypothetical protein -
  KFS84_RS15410 (KFS84_15345) - 3453716..3453961 (-) 246 WP_237654532.1 hypothetical protein -
  KFS84_RS15415 (KFS84_15350) - 3454022..3454978 (+) 957 WP_009597007.1 IS30 family transposase -
  KFS84_RS15420 (KFS84_15355) pilA/pilAI 3455037..3455447 (-) 411 WP_039955568.1 pilin Machinery gene
  KFS84_RS15425 (KFS84_15360) pilC 3455834..3457093 (+) 1260 WP_009580410.1 type II secretion system F family protein Machinery gene
  KFS84_RS15430 (KFS84_15365) - 3457100..3457963 (+) 864 WP_009580408.1 prepilin peptidase -
  KFS84_RS15435 (KFS84_15370) coaE 3457977..3458597 (+) 621 WP_009580406.1 dephospho-CoA kinase -
  KFS84_RS15440 (KFS84_15375) - 3458720..3460054 (-) 1335 WP_009580404.1 sensor histidine kinase -
  KFS84_RS15445 (KFS84_15380) - 3460047..3460724 (-) 678 WP_003466405.1 response regulator transcription factor -
  KFS84_RS15450 - 3460823..3461017 (-) 195 WP_231103461.1 hypothetical protein -
  KFS84_RS15455 (KFS84_15390) rimK 3461457..3462350 (-) 894 WP_009580399.1 30S ribosomal protein S6--L-glutamate ligase -
  KFS84_RS15460 (KFS84_15395) - 3462649..3464946 (+) 2298 WP_009580397.1 TonB-dependent receptor family protein -
  KFS84_RS15470 (KFS84_15405) - 3465924..3466687 (+) 764 WP_100225433.1 IS5 family transposase -
  KFS84_RS15475 (KFS84_15410) - 3466697..3467430 (+) 734 WP_237654616.1 IS5 family transposase -
  KFS84_RS15485 (KFS84_15420) - 3468940..3469821 (-) 882 WP_160317294.1 DUF4760 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14145.58 Da        Isoelectric Point: 8.4919

>NTDB_id=574087 KFS84_RS15420 WP_039955568.1 3455037..3455447(-) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain LMG 726]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVTAGLAEINPGKAQYEVALNEGKTAITGIGDLGLKSPTDRCT
ITDITALSPTGSIVCTLKGNTQVLGKTVTLKRNTDGSWTCATTVLAKHAPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=574087 KFS84_RS15420 WP_039955568.1 3455037..3455447(-) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain LMG 726]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTCATTGCGATCATCGCCATTTTGGCCGCCATTGCGCT
GCCGATGTACCAGGATTATGTCGCCAAGTCGCAAGTAACCGCTGGTCTTGCTGAGATCAACCCGGGTAAGGCCCAGTACG
AAGTTGCATTGAACGAAGGTAAGACCGCTATTACCGGCATTGGCGATCTTGGTTTGAAGTCTCCTACTGACCGTTGCACT
ATCACGGACATTACTGCGCTGAGTCCCACGGGAAGCATCGTTTGTACTTTGAAGGGTAACACCCAGGTTCTCGGCAAGAC
GGTTACCCTGAAACGTAATACTGATGGTTCCTGGACTTGTGCTACCACTGTTTTGGCGAAGCACGCTCCGGCCGGTTGCC
CGGGTGTTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M4L1M0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

52.593

99.265

0.522
  pilA Acinetobacter baumannii strain A118

51.095

100

0.515
  pilA Pseudomonas aeruginosa PAK

40.94

100

0.449
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.522

98.529

0.449
  pilA2 Legionella pneumophila str. Paris

43.796

100

0.441
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.434
  pilA Vibrio cholerae C6706

41.429

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.429

100

0.426
  pilA Vibrio cholerae strain A1552

41.429

100

0.426
  pilE Neisseria gonorrhoeae MS11

40.741

99.265

0.404
  comP Acinetobacter baylyi ADP1

35.57

100

0.39