Detailed information NTG0574088

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: KFS84_RS15425
• Genome accession: NZ_CP076254
• Coordinates: 3455834..3457093 (+)
• Length: 419 a.a.
• NCBI ID: WP_009580410.1
• Uniprot ID: A0A1M4JFC5
• Organism: Xanthomonas translucens pv. graminis strain LMG 726
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3449491..3470766	3455834..3457093	within	0
IS/Tn	3454022..3454978	3455834..3457093	flank	856

Gene organization within MGE regions

Location: 3449491..3470766

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KFS84_RS15375 (KFS84_15315)	-	3449491..3449787 (+)	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KFS84_RS15380 (KFS84_15320)	-	3449790..3450083 (+)	294	WP_009580422.1	addiction module antidote protein	-
KFS84_RS15385 (KFS84_15325)	pilB	3450111..3451847 (-)	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KFS84_RS15390 (KFS84_15330)	-	3452054..3452344 (+)	291	WP_009580418.1	nucleotidyltransferase family protein	-
KFS84_RS15395 (KFS84_15335)	-	3452341..3452661 (+)	321	WP_009580416.1	DUF86 domain-containing protein	-
KFS84_RS15400	-	3452761..3453012 (+)	252	WP_009580414.1	transposase	-
KFS84_RS15405	-	3453013..3453318 (+)	306	WP_231103532.1	hypothetical protein	-
KFS84_RS15410 (KFS84_15345)	-	3453716..3453961 (-)	246	WP_237654532.1	hypothetical protein	-
KFS84_RS15415 (KFS84_15350)	-	3454022..3454978 (+)	957	WP_009597007.1	IS30 family transposase	-
KFS84_RS15420 (KFS84_15355)	pilA/pilAI	3455037..3455447 (-)	411	WP_039955568.1	pilin	Machinery gene
KFS84_RS15425 (KFS84_15360)	pilC	3455834..3457093 (+)	1260	WP_009580410.1	type II secretion system F family protein	Machinery gene
KFS84_RS15430 (KFS84_15365)	-	3457100..3457963 (+)	864	WP_009580408.1	prepilin peptidase	-
KFS84_RS15435 (KFS84_15370)	coaE	3457977..3458597 (+)	621	WP_009580406.1	dephospho-CoA kinase	-
KFS84_RS15440 (KFS84_15375)	-	3458720..3460054 (-)	1335	WP_009580404.1	sensor histidine kinase	-
KFS84_RS15445 (KFS84_15380)	-	3460047..3460724 (-)	678	WP_003466405.1	response regulator transcription factor	-
KFS84_RS15450	-	3460823..3461017 (-)	195	WP_231103461.1	hypothetical protein	-
KFS84_RS15455 (KFS84_15390)	rimK	3461457..3462350 (-)	894	WP_009580399.1	30S ribosomal protein S6--L-glutamate ligase	-
KFS84_RS15460 (KFS84_15395)	-	3462649..3464946 (+)	2298	WP_009580397.1	TonB-dependent receptor family protein	-
KFS84_RS15470 (KFS84_15405)	-	3465924..3466687 (+)	764	WP_100225433.1	IS5 family transposase	-
KFS84_RS15475 (KFS84_15410)	-	3466697..3467430 (+)	734	WP_237654616.1	IS5 family transposase	-
KFS84_RS15485 (KFS84_15420)	-	3468940..3469821 (-)	882	WP_160317294.1	DUF4760 domain-containing protein	-

Sequence

Protein

• Length: 419 a.a.
• Molecular weight: 45494.63 Da
• Isoelectric Point: 10.1825

>NTDB_id=574088 KFS84_RS15425 WP_009580410.1 3455834..3457093(+) (pilC) [Xanthomonas translucens pv. graminis strain LMG 726]
MSATRSAVSKEPVARNTSQQVPFVWEGTDKRGIKMKGEQTAKNANLLRAELRRQGITPSVVKLKPKPLFGAAGSKISAKD
IAFFSRQMATMMKSGVPIVGSLEIIGSGHKNPRMKKMVGQVRTDIEGGSSLYEAISKHPVQFDELYRNLVKAGEGAGVLE
TVLETVATYKENTEALKGKIKKALFYPAAVMGVALLVSSILLVWVVPQFEDVFKGFGAELPAFTQMIVAASRFMVAYWWL
LLIVLVGSIVGFVFAYKRSPSMQHGMDRLILKVPIIGQIMHNSSVARFSRTLGVTFRAGVPLVEALDIVAGATGNSVYEK
AVLRMRDDVSVGYPVNVAMKQANLFPHMVIQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIV
GGMVVGMYLPIFKLASVVG

Nucleotide

• Length: 1260 bp

>NTDB_id=574088 KFS84_RS15425 WP_009580410.1 3455834..3457093(+) (pilC) [Xanthomonas translucens pv. graminis strain LMG 726]
ATGTCCGCAACTCGTAGCGCAGTATCCAAAGAACCCGTCGCACGCAATACCAGCCAGCAGGTCCCGTTCGTCTGGGAAGG
GACGGACAAGCGCGGCATCAAGATGAAGGGCGAGCAGACCGCCAAGAACGCCAACCTGCTGCGTGCGGAATTGCGCCGCC
AGGGCATCACGCCGTCGGTGGTCAAGCTGAAACCCAAGCCCTTGTTCGGTGCCGCCGGCAGCAAGATCAGCGCGAAGGAC
ATTGCCTTCTTCAGCCGCCAGATGGCGACCATGATGAAGTCGGGCGTCCCCATCGTGGGATCGCTGGAGATCATCGGCAG
CGGGCACAAGAACCCGCGCATGAAGAAGATGGTGGGACAGGTCCGCACGGACATCGAAGGCGGCTCGTCGCTCTATGAAG
CGATCAGCAAGCATCCGGTCCAGTTCGATGAGCTCTACCGCAACCTGGTCAAGGCCGGCGAAGGCGCCGGTGTACTCGAA
ACCGTGCTGGAGACGGTAGCCACCTACAAGGAAAACACCGAGGCGCTGAAGGGCAAGATCAAGAAAGCCTTGTTCTATCC
GGCCGCGGTCATGGGTGTGGCGCTCCTGGTCAGCTCGATCTTGCTGGTGTGGGTGGTCCCGCAATTCGAGGATGTCTTCA
AAGGGTTTGGCGCGGAGTTACCCGCATTCACTCAGATGATCGTCGCCGCTTCACGCTTTATGGTGGCTTATTGGTGGCTC
CTGCTGATCGTGCTGGTTGGCAGCATCGTCGGCTTCGTCTTCGCCTACAAGCGCTCGCCGTCGATGCAGCACGGCATGGA
CCGACTGATCCTGAAGGTGCCGATCATCGGCCAGATCATGCACAACAGCTCCGTGGCCCGCTTCTCGCGGACACTGGGCG
TCACCTTCCGCGCCGGCGTCCCGTTGGTGGAAGCGCTGGACATCGTCGCCGGCGCCACCGGCAACAGCGTCTACGAGAAA
GCCGTGCTGCGCATGCGCGACGACGTGTCGGTGGGCTACCCGGTCAATGTGGCGATGAAACAGGCCAACCTGTTTCCGCA
CATGGTCATCCAGATGACCGCCATCGGCGAAGAAGCCGGTGCGCTGGATGCGATGCTGTTCAAGGTGGCCGAGTACTTCG
AGCAGGAAGTGAACAATGCGGTCGATGCGTTGAGCAGCCTGCTAGAACCGCTGATCATGGTGTTCATTGGTACCATCGTC
GGCGGCATGGTCGTCGGCATGTATCTGCCCATCTTCAAGCTGGCCTCCGTCGTTGGATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (84-207)

• Type II secretion system (T2SS), protein F (T2SSF) (288-410)

3D structure

• AlphaFold DB: A0A1M4JFC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	53.535	94.511	0.506
pilC	Legionella pneumophila strain ERS1305867	51.733	96.42	0.499
pilC	Acinetobacter baylyi ADP1	50.735	97.375	0.494
pilC	Acinetobacter baumannii D1279779	50.245	97.375	0.489
pilC	Vibrio cholerae strain A1552	42.222	96.659	0.408
pilG	Neisseria gonorrhoeae MS11	42.714	94.988	0.406
pilG	Neisseria meningitidis 44/76-A	42.105	95.227	0.401
pilC	Vibrio campbellii strain DS40M4	40.05	94.749	0.379
pilC	Thermus thermophilus HB27	38.75	95.465	0.37