Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KI608_RS04055 Genome accession   NZ_CP075192
Coordinates   839516..839947 (+) Length   143 a.a.
NCBI ID   WP_319004137.1    Uniprot ID   -
Organism   Ferribacterium limneticum strain 132     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 840047..854652 839516..839947 flank 100


Gene organization within MGE regions


Location: 839516..854652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI608_RS04055 (KI608_04055) pilE 839516..839947 (+) 432 WP_319004137.1 pilin Machinery gene
  KI608_RS04060 (KI608_04060) - 840005..841963 (+) 1959 WP_226450743.1 hypothetical protein -
  KI608_RS04065 (KI608_04065) - 841975..842865 (+) 891 WP_226451835.1 glycosyltransferase family 2 protein -
  KI608_RS04070 (KI608_04070) - 842879..844285 (+) 1407 WP_226450744.1 MBOAT family protein -
  KI608_RS04075 (KI608_04075) - 844260..845357 (+) 1098 WP_226450745.1 hypothetical protein -
  KI608_RS04080 (KI608_04080) - 845354..846475 (+) 1122 WP_226450746.1 glycosyltransferase family 4 protein -
  KI608_RS04085 (KI608_04085) - 846472..847374 (+) 903 WP_226450747.1 NAD-dependent epimerase/dehydratase family protein -
  KI608_RS04090 (KI608_04090) - 847371..848387 (+) 1017 WP_226450748.1 glycosyltransferase family 2 protein -
  KI608_RS04095 (KI608_04095) - 848418..849668 (-) 1251 WP_226450749.1 glycoside hydrolase family 99-like domain-containing protein -
  KI608_RS04100 (KI608_04100) - 849812..850426 (-) 615 WP_226450750.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  KI608_RS04105 (KI608_04105) - 850419..851111 (-) 693 WP_226450751.1 NTP transferase domain-containing protein -
  KI608_RS04110 (KI608_04110) - 851108..851992 (-) 885 WP_226450752.1 hypothetical protein -
  KI608_RS04115 (KI608_04115) - 851989..852615 (-) 627 WP_226450753.1 hypothetical protein -
  KI608_RS04120 (KI608_04120) - 852618..853877 (-) 1260 WP_226450754.1 ABC transporter ATP-binding protein -
  KI608_RS04125 (KI608_04125) - 853867..854652 (-) 786 WP_226450755.1 ABC transporter permease -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14588.02 Da        Isoelectric Point: 9.4205

>NTDB_id=568598 KI608_RS04055 WP_319004137.1 839516..839947(+) (pilE) [Ferribacterium limneticum strain 132]
MKNMQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSELILASSAPKVSIAEWFQTKGSMPPSATSAGFDVTGSKY
VSGITYAVAGNVATITVAAQAIGGSTPTGTILMTGTGDTTTGKVAWACTVGTMAAKYVPSSCK

Nucleotide


Download         Length: 432 bp        

>NTDB_id=568598 KI608_RS04055 WP_319004137.1 839516..839947(+) (pilE) [Ferribacterium limneticum strain 132]
ATGAAGAATATGCAAAAAGGTTTTACCCTGATCGAACTGATGATTGTTGTCGCCATTATCGGTATTCTGGCTGCAGTTGC
GTTGCCGGCGTATCAGGACTACACGGTGCGTGCAAAGGTTAGCGAACTGATTCTTGCCTCTTCAGCCCCGAAGGTTTCAA
TTGCCGAGTGGTTCCAAACCAAAGGCTCGATGCCTCCCTCCGCCACTTCTGCCGGCTTTGATGTTACTGGGTCAAAATAC
GTTTCTGGCATAACCTACGCCGTGGCTGGCAATGTCGCTACCATTACGGTAGCTGCGCAAGCTATTGGCGGTAGCACGCC
GACTGGTACGATTCTGATGACGGGAACCGGTGACACGACAACAGGAAAGGTCGCTTGGGCATGTACCGTAGGCACGATGG
CTGCCAAGTACGTACCTTCAAGCTGCAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.105

100

0.559
  pilA2 Legionella pneumophila strain ERS1305867

51.064

98.601

0.503
  pilA2 Legionella pneumophila str. Paris

48.276

100

0.49
  comP Acinetobacter baylyi ADP1

44.667

100

0.469
  pilE Neisseria gonorrhoeae MS11

39.634

100

0.455
  pilE Neisseria gonorrhoeae strain FA1090

38.889

100

0.441
  pilA Ralstonia pseudosolanacearum GMI1000

38.75

100

0.434
  pilA/pilA1 Eikenella corrodens VA1

36.943

100

0.406
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

100

0.399
  pilA Vibrio parahaemolyticus RIMD 2210633

42.636

90.21

0.385
  pilA Pseudomonas aeruginosa PAK

36.913

100

0.385
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.589

98.601

0.371