Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   J5226_RS09750 Genome accession   NZ_CP072597
Coordinates   2259409..2259831 (-) Length   140 a.a.
NCBI ID   WP_215839719.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2254409..2264831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS09730 (J5226_09730) - 2254681..2255763 (+) 1083 WP_215839715.1 UDP-N-acetylglucosamine 2-epimerase -
  J5226_RS09735 (J5226_09735) - 2255846..2256769 (+) 924 WP_215839716.1 hypothetical protein -
  J5226_RS09740 (J5226_09740) - 2256808..2258724 (-) 1917 WP_215839717.1 tetratricopeptide repeat protein -
  J5226_RS09745 (J5226_09745) - 2258825..2259241 (-) 417 WP_215839718.1 pilin -
  J5226_RS09750 (J5226_09750) pilA2 2259409..2259831 (-) 423 WP_215839719.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5226_RS09755 (J5226_09755) pilA2 2260711..2261133 (-) 423 WP_215839720.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5226_RS09760 (J5226_09760) pilR 2261801..2263216 (-) 1416 WP_215839721.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14938.21 Da        Isoelectric Point: 8.0997

>NTDB_id=554461 J5226_RS09750 WP_215839719.1 2259409..2259831(-) (pilA2) [Lysobacter sp. K5869]
MKKQQGFTLIELMIVIAILGILIAIALPAYQDYTVRTKNAECLNVAAAAKLAVSETAQDRGSLAAITSQSITGYEFKASD
YCKDVKIAANGVIQTEAQATGADKMAKFDLTPAFSAGRIDWTCKETNGSKKSQLPAECRG

Nucleotide


Download         Length: 423 bp        

>NTDB_id=554461 J5226_RS09750 WP_215839719.1 2259409..2259831(-) (pilA2) [Lysobacter sp. K5869]
ATGAAGAAGCAGCAAGGCTTTACCCTGATCGAACTGATGATCGTCATCGCGATCCTCGGCATCCTGATCGCCATCGCTCT
GCCGGCCTACCAGGACTACACCGTCCGCACCAAGAACGCCGAGTGCCTGAACGTCGCCGCCGCCGCCAAGCTGGCCGTGT
CCGAAACCGCTCAGGACCGTGGCTCGCTCGCCGCCATCACCTCCCAGTCGATCACCGGCTATGAGTTCAAGGCCAGCGAC
TACTGCAAGGACGTCAAGATCGCCGCCAACGGCGTGATCCAGACCGAAGCCCAGGCCACTGGCGCCGACAAGATGGCGAA
GTTCGATCTGACCCCGGCTTTCAGCGCCGGTCGCATCGACTGGACCTGCAAGGAAACCAACGGTTCCAAGAAGTCGCAGC
TGCCGGCCGAGTGCCGCGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

48.227

100

0.486
  pilA2 Legionella pneumophila strain ERS1305867

47.857

100

0.479
  comP Acinetobacter baylyi ADP1

43.709

100

0.471
  pilA Ralstonia pseudosolanacearum GMI1000

37.805

100

0.443
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40

100

0.443
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.304

98.571

0.407
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.4
  pilE Neisseria gonorrhoeae strain FA1090

35.484

100

0.393
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.686

97.857

0.379
  pilA Haemophilus influenzae 86-028NP

34.932

100

0.364