Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   J5226_RS09755 Genome accession   NZ_CP072597
Coordinates   2260711..2261133 (-) Length   140 a.a.
NCBI ID   WP_215839720.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2255711..2266133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS09735 (J5226_09735) - 2255846..2256769 (+) 924 WP_215839716.1 hypothetical protein -
  J5226_RS09740 (J5226_09740) - 2256808..2258724 (-) 1917 WP_215839717.1 tetratricopeptide repeat protein -
  J5226_RS09745 (J5226_09745) - 2258825..2259241 (-) 417 WP_215839718.1 pilin -
  J5226_RS09750 (J5226_09750) pilA2 2259409..2259831 (-) 423 WP_215839719.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5226_RS09755 (J5226_09755) pilA2 2260711..2261133 (-) 423 WP_215839720.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J5226_RS09760 (J5226_09760) pilR 2261801..2263216 (-) 1416 WP_215839721.1 sigma-54 dependent transcriptional regulator Regulator
  J5226_RS09765 (J5226_09765) - 2263314..2264939 (-) 1626 WP_215839722.1 ATP-binding protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14932.28 Da        Isoelectric Point: 8.8256

>NTDB_id=554462 J5226_RS09755 WP_215839720.1 2260711..2261133(-) (pilA2) [Lysobacter sp. K5869]
MNKQQGFTLIELMIVIAILGILIAIALPAYQDYTVRTKNAECLNIAAAAKLAVSETAQDRGSLAAITTQSVTGYQFAPSK
YCKNVAIIKDGVITTEAQKTGATTEAKFKLEPTFSAGRIDWKCTDTGGAKKSQLPAECRG

Nucleotide


Download         Length: 423 bp        

>NTDB_id=554462 J5226_RS09755 WP_215839720.1 2260711..2261133(-) (pilA2) [Lysobacter sp. K5869]
ATGAACAAGCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTCATCGCGATTCTCGGCATCCTGATCGCCATCGCGTT
GCCGGCGTATCAGGACTACACCGTCCGCACCAAGAACGCCGAGTGCTTGAACATCGCGGCGGCCGCCAAGCTGGCCGTGT
CCGAAACGGCTCAGGACCGTGGCTCGCTGGCCGCCATCACCACCCAGTCCGTCACCGGCTATCAGTTCGCGCCCAGCAAG
TACTGCAAGAATGTCGCGATCATCAAGGATGGCGTCATCACCACCGAGGCTCAGAAGACCGGCGCTACCACGGAGGCCAA
GTTCAAGCTCGAGCCGACCTTCAGCGCAGGCCGCATCGATTGGAAGTGCACCGACACCGGCGGCGCCAAGAAGTCGCAGC
TGCCGGCCGAGTGCAGGGGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

48.571

100

0.486
  pilA2 Legionella pneumophila str. Paris

48.571

100

0.486
  comP Acinetobacter baylyi ADP1

44.079

100

0.479
  pilA Ralstonia pseudosolanacearum GMI1000

38.323

100

0.457
  pilE Neisseria gonorrhoeae strain FA1090

38.065

100

0.421
  pilA/pilA1 Eikenella corrodens VA1

37.5

100

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

100

0.4
  pilE Neisseria gonorrhoeae MS11

42.52

90.714

0.386