Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSEST_RS15965 Genome accession   NC_019936
Coordinates   3452595..3453812 (-) Length   405 a.a.
NCBI ID   WP_015278009.1    Uniprot ID   L0GLZ5
Organism   Stutzerimonas stutzeri RCH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3450781..3458914 3452595..3453812 within 0


Gene organization within MGE regions


Location: 3450781..3458914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEST_RS21875 (Psest_3284) yacG 3450781..3450975 (-) 195 WP_015278006.1 DNA gyrase inhibitor YacG -
  PSEST_RS15955 (Psest_3285) coaE 3450972..3451580 (-) 609 WP_015278007.1 dephospho-CoA kinase -
  PSEST_RS15960 (Psest_3286) pilD 3451723..3452592 (-) 870 WP_015278008.1 prepilin peptidase Machinery gene
  PSEST_RS15965 (Psest_3287) pilC 3452595..3453812 (-) 1218 WP_015278009.1 type II secretion system F family protein Machinery gene
  PSEST_RS15970 (Psest_3288) pilB 3453814..3455517 (-) 1704 WP_015278010.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEST_RS15975 (Psest_3289) pilA/pilAI 3455850..3456266 (+) 417 WP_419184897.1 pilin Machinery gene
  PSEST_RS15980 (Psest_3290) pilA/pilAII 3456335..3456751 (+) 417 WP_015278012.1 pilin Regulator
  PSEST_RS15985 (Psest_3291) - 3456711..3458168 (+) 1458 WP_015278013.1 O-antigen ligase family protein -
  PSEST_RS15990 (Psest_3292) tfpZ 3458177..3458914 (+) 738 WP_015278014.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44025.73 Da        Isoelectric Point: 9.9472

>NTDB_id=55412 PSEST_RS15965 WP_015278009.1 3452595..3453812(-) (pilC) [Stutzerimonas stutzeri RCH2]
MAQKAIKTSVFTWEGTNRQGAKIKGELSGVSPALVKAQLRKQGVNPQKVRKKSVSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDLKQEVAAGNSFATALRKKPQYFDDLYCNLVDSGEQSGSLETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLVAIIVSAILLIKVVPQFQDVFANFGAELPAFTLMVIGLSEALQAWWHVVLFVMFGVAYAFK
TAHGKSERFRNGFDRFLLRIPVVGDILYKSAVARFARTLATTFAAGVPLVDALDSVAGATGNVVFRNATMKVKSDVSSGM
QLNFSMRTTGTFPTMAVQMTAIGEESGALDEMLGKVATFYEDEVDNMVDGLTSLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=55412 PSEST_RS15965 WP_015278009.1 3452595..3453812(-) (pilC) [Stutzerimonas stutzeri RCH2]
ATGGCGCAGAAAGCGATAAAGACCAGTGTGTTCACCTGGGAAGGCACAAACAGGCAAGGCGCAAAGATAAAGGGTGAGCT
TAGCGGCGTTAGCCCCGCGCTGGTCAAGGCGCAGCTGCGCAAGCAGGGTGTCAACCCGCAGAAGGTGCGCAAGAAGTCGG
TTTCATTGTTTGGTGCTGGCAAGAAGATCAAACCGATGGATATTGCCCTGTTCACTCGGCAGATGGCGACCATGATGAAG
GCGGGCGTTCCGCTCTTGCAGTCCTTCGACATCATTGGTGAGGGCTTTGATAATCCGAATATGCGCAAACTGGTGGATGA
TTTGAAGCAGGAAGTCGCTGCGGGCAATAGCTTCGCAACCGCACTGCGAAAGAAGCCTCAGTACTTCGATGATCTTTATT
GCAATCTAGTGGATTCTGGTGAGCAATCCGGCTCGCTGGAAACGCTCCTTGATAGGGTGGCGACCTACAAGGAAAAGACC
GAGGCCCTGAAGGCCAAGATCAAGAAGGCGATGAACTACCCAATCGCGGTAGTGCTGGTTGCGATCATTGTGTCCGCGAT
CCTGTTGATCAAGGTGGTTCCGCAGTTTCAGGATGTTTTCGCAAACTTCGGCGCGGAGCTTCCGGCGTTCACATTGATGG
TGATTGGCTTGTCCGAAGCACTGCAGGCCTGGTGGCATGTGGTGCTGTTCGTTATGTTTGGGGTGGCTTATGCCTTCAAA
ACAGCCCACGGCAAATCGGAACGTTTTCGCAACGGGTTTGACCGCTTTTTGCTCAGAATTCCCGTCGTCGGCGATATTCT
CTACAAGTCTGCAGTGGCACGCTTTGCGCGCACGCTGGCTACTACCTTTGCTGCTGGTGTGCCGTTGGTTGATGCGTTGG
ATTCAGTCGCCGGCGCCACTGGCAATGTGGTGTTCCGCAATGCAACGATGAAAGTGAAGAGTGACGTTTCGAGTGGTATG
CAGCTCAACTTCTCCATGCGTACAACTGGCACCTTCCCTACCATGGCGGTTCAGATGACCGCCATTGGCGAAGAGTCCGG
CGCGCTCGATGAGATGCTGGGGAAAGTCGCGACGTTTTACGAGGACGAAGTTGACAATATGGTCGATGGCCTGACAAGTC
TCATGGAGCCAATGATTATGGCTGTGCTGGGTGTTTTGGTTGGCGGCTTGATTATCGCTATGTACCTTCCGATTTTTCAG
CTTGGTTCTGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GLZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

91.605

100

0.916
  pilC Acinetobacter baylyi ADP1

60.099

100

0.602
  pilC Acinetobacter baumannii D1279779

59.259

100

0.593
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

44.169

99.506

0.44
  pilG Neisseria meningitidis 44/76-A

44.169

99.506

0.44
  pilC Vibrio cholerae strain A1552

42.424

97.778

0.415
  pilC Vibrio campbellii strain DS40M4

41.414

97.778

0.405
  pilC Thermus thermophilus HB27

36.842

98.519

0.363

Multiple sequence alignment