Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSEST_RS15970 Genome accession   NC_019936
Coordinates   3453814..3455517 (-) Length   567 a.a.
NCBI ID   WP_015278010.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri RCH2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3450781..3458914 3453814..3455517 within 0


Gene organization within MGE regions


Location: 3450781..3458914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEST_RS21875 (Psest_3284) yacG 3450781..3450975 (-) 195 WP_015278006.1 DNA gyrase inhibitor YacG -
  PSEST_RS15955 (Psest_3285) coaE 3450972..3451580 (-) 609 WP_015278007.1 dephospho-CoA kinase -
  PSEST_RS15960 (Psest_3286) pilD 3451723..3452592 (-) 870 WP_015278008.1 prepilin peptidase Machinery gene
  PSEST_RS15965 (Psest_3287) pilC 3452595..3453812 (-) 1218 WP_015278009.1 type II secretion system F family protein Machinery gene
  PSEST_RS15970 (Psest_3288) pilB 3453814..3455517 (-) 1704 WP_015278010.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEST_RS15975 (Psest_3289) pilA/pilAI 3455850..3456266 (+) 417 WP_419184897.1 pilin Machinery gene
  PSEST_RS15980 (Psest_3290) pilA/pilAII 3456335..3456751 (+) 417 WP_015278012.1 pilin Regulator
  PSEST_RS15985 (Psest_3291) - 3456711..3458168 (+) 1458 WP_015278013.1 O-antigen ligase family protein -
  PSEST_RS15990 (Psest_3292) tfpZ 3458177..3458914 (+) 738 WP_015278014.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61786.72 Da        Isoelectric Point: 5.2370

>NTDB_id=55413 PSEST_RS15970 WP_015278010.1 3453814..3455517(-) (pilB) [Stutzerimonas stutzeri RCH2]
MSENISLSGLARQVVGAGVLDERTAQQAQAQALRSKTPFVTYAVQNRLVNGRVIAELASEQFGVALLDLGALDKELLPKD
LISEKLAKQHRVLPLSKRGTKLFIAISDPANHQSIADIQFSTGLSIEPILVEDDKLGEAIDKLYDNGSTGLEDLGDVDLD
GVDVESVSDDGKGDEAGETADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRRPQDGRIKMKLSKNKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLGEGFP
ADKIGAFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=55413 PSEST_RS15970 WP_015278010.1 3453814..3455517(-) (pilB) [Stutzerimonas stutzeri RCH2]
ATGAGCGAAAATATTTCTCTATCCGGGCTGGCTCGGCAGGTGGTCGGTGCCGGGGTGCTAGACGAGAGAACGGCACAGCA
GGCCCAAGCCCAGGCGCTTCGCAGCAAAACGCCCTTCGTTACCTATGCCGTTCAGAACCGGCTGGTCAATGGGCGAGTCA
TAGCCGAGCTTGCGTCCGAGCAGTTTGGCGTCGCATTGCTGGATCTGGGAGCGCTGGATAAGGAGCTGCTGCCCAAGGAT
CTGATTAGTGAGAAACTTGCCAAGCAGCATCGTGTGCTCCCCCTTTCCAAACGTGGAACCAAGCTGTTCATCGCTATTTC
CGACCCTGCGAATCATCAGTCGATTGCCGATATCCAGTTCAGTACCGGGCTTTCGATTGAGCCGATATTGGTGGAAGACG
ATAAGCTCGGCGAGGCAATCGACAAACTATACGACAACGGTTCTACCGGACTGGAGGATCTAGGTGACGTTGACCTGGAT
GGCGTAGATGTCGAATCGGTTAGTGATGATGGCAAGGGTGACGAGGCCGGAGAGACTGCAGACGATGCGCCAGTGGTTCG
TTTCGTCAACAAAATGCTGCTCGACGCCATTCGTGGGGGCTCTTCCGACCTGCATTTCGAGCCTTATGAGAAGGCGTTTC
GCGTGCGGCTGCGTACCGATGGCATCCTGCATGAGGTGGCACGTCCGCCGATACAGCTGGCGCCTAGGATTTCCGCGCGC
CTCAAAGTGATGGCGGGCTTGGATATTTCCGAGCGTCGTAGACCTCAGGATGGCCGCATCAAGATGAAGCTGTCCAAGAA
CAAGGCGATTGATTTTCGCGTCAATACGCTGCCTACGCTGTGGGGCGAGAAGATCGTGATGCGAATCCTCGATCCGTCCA
GCGCACAGATGGGTATTGATGCATTGGGTTATGAAGAGGATCAGAAAGAGCTTTATATGAATGCCCTGAAGCAACCACAG
GGCATGATTTTGGTCACCGGCCCGACTGGCTCTGGTAAGACTGTGTCCCTCTATACGGGGTTGAACATCCTTAATACGGT
CGATGTGAATATCTCTACCGCAGAAGATCCTGTGGAGATCAATCTGGAAGGCATCAATCAAGTCAACGTCAATCCCCGCC
AGGGGATGGATTTTTCGCAGGCGCTACGCGCGTTTCTGCGTCAGGATCCGGACATCATTATGGTGGGTGAGATCCGTGAT
CTGGAGACCGCTGAGATTGCCATCAAGGCGGCGCAGACCGGTCACATGGTCATGTCTACTCTCCATACCAACAGCGCAGC
AGAAACCCTCACGCGACTTCGTAACATGGGGGTGGCCGCGTTCAATATCGCGACATCGGTCAACCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGCTGCAAGAAAGAGGTTGAGCTGCCGCGCGAGGCGCTGCTGGGCGAAGGTTTTCCT
GCCGACAAGATTGGGGCATTCAAAATTTATGGCCCGGTCGGCTGCGATAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCTCTCGCGAGGATTATCATGGAGGACGGCAATTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTTAACGATCTGCGTACTTCGGCTCTAGTTAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.295

0.587
  pilB Acinetobacter baumannii D1279779

58.688

99.471

0.584
  pilB Legionella pneumophila strain ERS1305867

54.545

100

0.55
  pilF Neisseria gonorrhoeae MS11

48.673

99.647

0.485
  pilB Vibrio cholerae strain A1552

52.216

91.534

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

51.938

91.005

0.473
  pilB Vibrio campbellii strain DS40M4

50.288

91.887

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

35.563

100

0.384

Multiple sequence alignment