Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAII   Type   Regulator
Locus tag   PSEST_RS15980 Genome accession   NC_019936
Coordinates   3456335..3456751 (+) Length   138 a.a.
NCBI ID   WP_015278012.1    Uniprot ID   L0GP32
Organism   Stutzerimonas stutzeri RCH2     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3450781..3458914 3456335..3456751 within 0


Gene organization within MGE regions


Location: 3450781..3458914
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEST_RS21875 (Psest_3284) yacG 3450781..3450975 (-) 195 WP_015278006.1 DNA gyrase inhibitor YacG -
  PSEST_RS15955 (Psest_3285) coaE 3450972..3451580 (-) 609 WP_015278007.1 dephospho-CoA kinase -
  PSEST_RS15960 (Psest_3286) pilD 3451723..3452592 (-) 870 WP_015278008.1 prepilin peptidase Machinery gene
  PSEST_RS15965 (Psest_3287) pilC 3452595..3453812 (-) 1218 WP_015278009.1 type II secretion system F family protein Machinery gene
  PSEST_RS15970 (Psest_3288) pilB 3453814..3455517 (-) 1704 WP_015278010.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSEST_RS15975 (Psest_3289) pilA/pilAI 3455850..3456266 (+) 417 WP_419184897.1 pilin Machinery gene
  PSEST_RS15980 (Psest_3290) pilA/pilAII 3456335..3456751 (+) 417 WP_015278012.1 pilin Regulator
  PSEST_RS15985 (Psest_3291) - 3456711..3458168 (+) 1458 WP_015278013.1 O-antigen ligase family protein -
  PSEST_RS15990 (Psest_3292) tfpZ 3458177..3458914 (+) 738 WP_015278014.1 TfpX/TfpZ family type IV pilin accessory protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14527.79 Da        Isoelectric Point: 9.1920

>NTDB_id=55415 PSEST_RS15980 WP_015278012.1 3456335..3456751(+) (pilA/pilAII) [Stutzerimonas stutzeri RCH2]
MKKSYRGFSLIELMTALSIIGILAAIAFPAYQNYTVRSTAAAALAEITPAKAAFEHAISENRTPSLVSSLPGFIGIGAST
NYCTVTLIAAATGSITCTARNGMPGRFNGKIITLNRTAEGLWSCTSDLDSQYKPGKCL

Nucleotide


Download         Length: 417 bp        

>NTDB_id=55415 PSEST_RS15980 WP_015278012.1 3456335..3456751(+) (pilA/pilAII) [Stutzerimonas stutzeri RCH2]
GTGAAAAAGTCGTACCGCGGCTTTTCTCTGATTGAATTGATGACCGCGCTATCAATCATCGGCATTCTTGCGGCCATTGC
ATTTCCCGCCTATCAGAACTACACCGTGCGCTCGACAGCTGCAGCCGCCTTGGCCGAAATCACTCCGGCAAAAGCCGCGT
TCGAGCACGCCATTAGCGAAAATCGCACGCCTTCACTGGTGTCCAGCTTGCCGGGATTTATCGGTATCGGCGCGAGCACA
AACTACTGCACGGTAACGCTAATAGCAGCGGCGACGGGATCGATCACCTGCACCGCTCGGAACGGAATGCCAGGGCGGTT
CAATGGCAAAATCATTACCCTTAACCGCACGGCGGAGGGCTTGTGGAGCTGTACCAGTGATCTGGACAGCCAATATAAGC
CGGGAAAATGCCTTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L0GP32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAII Pseudomonas stutzeri DSM 10701

83.333

100

0.833
  pilA/pilAI Pseudomonas stutzeri DSM 10701

66.667

95.652

0.638
  pilA Pseudomonas aeruginosa PAK

45.395

100

0.5
  comP Acinetobacter baylyi ADP1

40.541

100

0.435
  pilA Acinetobacter baumannii strain A118

40.146

99.275

0.399
  pilA2 Legionella pneumophila strain ERS1305867

38.686

99.275

0.384
  pilA2 Legionella pneumophila str. Paris

37.956

99.275

0.377

Multiple sequence alignment