Detailed information NTG0055411

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSEST_RS15960
• Genome accession: NC_019936
• Coordinates: 3451723..3452592 (-)
• Length: 289 a.a.
• NCBI ID: WP_015278008.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri RCH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3450781..3458914	3451723..3452592	within	0

Gene organization within MGE regions

Location: 3450781..3458914

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSEST_RS21875 (Psest_3284)	yacG	3450781..3450975 (-)	195	WP_015278006.1	DNA gyrase inhibitor YacG	-
PSEST_RS15955 (Psest_3285)	coaE	3450972..3451580 (-)	609	WP_015278007.1	dephospho-CoA kinase	-
PSEST_RS15960 (Psest_3286)	pilD	3451723..3452592 (-)	870	WP_015278008.1	prepilin peptidase	Machinery gene
PSEST_RS15965 (Psest_3287)	pilC	3452595..3453812 (-)	1218	WP_015278009.1	type II secretion system F family protein	Machinery gene
PSEST_RS15970 (Psest_3288)	pilB	3453814..3455517 (-)	1704	WP_015278010.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSEST_RS15975 (Psest_3289)	pilA/pilAI	3455850..3456266 (+)	417	WP_419184897.1	pilin	Machinery gene
PSEST_RS15980 (Psest_3290)	pilA/pilAII	3456335..3456751 (+)	417	WP_015278012.1	pilin	Regulator
PSEST_RS15985 (Psest_3291)	-	3456711..3458168 (+)	1458	WP_015278013.1	O-antigen ligase family protein	-
PSEST_RS15990 (Psest_3292)	tfpZ	3458177..3458914 (+)	738	WP_015278014.1	TfpX/TfpZ family type IV pilin accessory protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32290.35 Da
• Isoelectric Point: 6.9298

>NTDB_id=55411 PSEST_RS15960 WP_015278008.1 3451723..3452592(-) (pilD) [Stutzerimonas stutzeri RCH2]
MILLDYLASHVLAFVLSAAVLGLLVGSFLNVVIYRLPIMMQRDWRMQALEYLESPAEPVGERFNLLLPNSRCPHCNHQIR
SWENIPLVSWLALRGKCSSCRAPISCRYPLVELACGLLSGYVAWHFGFSWQAGAMLLLTWGLLAMSMIDVDHQLLPDVLV
LPLLWLGLILNNFGLFVSLESALWGAVAGYLSLWSVYWLFKVVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVG
AVLGSILLRMQRAESNTPIPFGPYLAIAGWIALLWGDWITESYLQFVRF

Nucleotide

• Length: 870 bp

>NTDB_id=55411 PSEST_RS15960 WP_015278008.1 3451723..3452592(-) (pilD) [Stutzerimonas stutzeri RCH2]
ATGATTCTGCTCGATTATTTGGCCAGCCACGTGCTGGCCTTTGTTTTATCTGCTGCCGTGCTCGGCTTATTGGTCGGCAG
CTTTCTAAATGTCGTAATTTACCGCCTCCCGATTATGATGCAGCGCGATTGGCGTATGCAGGCGCTTGAATATCTCGAGA
GCCCGGCGGAGCCAGTCGGCGAGCGCTTCAATCTGCTGCTGCCAAACTCCCGTTGTCCGCATTGCAATCACCAAATCCGC
TCTTGGGAAAATATCCCCCTAGTCAGCTGGTTGGCCCTGCGTGGCAAGTGCTCATCCTGCCGTGCGCCGATCAGTTGTCG
TTATCCGTTGGTGGAGTTGGCTTGCGGGCTGTTGTCCGGCTATGTCGCCTGGCATTTCGGCTTCTCCTGGCAGGCCGGCG
CAATGCTGTTGCTGACCTGGGGCCTGTTGGCGATGAGCATGATCGATGTCGATCACCAGTTGTTGCCTGACGTTTTGGTG
CTGCCGCTATTGTGGCTCGGCCTTATCCTCAATAACTTCGGTTTGTTCGTATCGTTGGAAAGTGCGCTATGGGGAGCAGT
AGCGGGGTACCTGAGTCTTTGGTCGGTGTACTGGCTGTTCAAGGTGGTCACCGGTAAAGAGGGTATGGGCTACGGTGACT
TCAAGCTGCTGGCCATGCTTGGCGCCTGGGGCGGGTGGCAGGTGCTGCCGCTGACGATCCTGTTGTCTTCGGTGGTCGGC
GCTGTGCTTGGTAGCATCCTCTTGCGGATGCAGCGTGCGGAAAGCAATACGCCGATTCCTTTCGGCCCATACCTGGCTAT
CGCAGGCTGGATTGCATTGCTTTGGGGCGATTGGATTACGGAAAGCTACCTGCAGTTTGTGCGCTTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.664	94.81	0.547
pilD	Vibrio campbellii strain DS40M4	56.044	94.464	0.529
pilD	Acinetobacter nosocomialis M2	52.113	98.27	0.512
pilD	Acinetobacter baumannii D1279779	52.113	98.27	0.512
pilD	Neisseria gonorrhoeae MS11	52.874	90.311	0.478