Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J7445_RS05900 Genome accession   NZ_CP072524
Coordinates   1253839..1254966 (+) Length   375 a.a.
NCBI ID   WP_003742946.1    Uniprot ID   A0A0C1GN56
Organism   Neisseria sicca strain NS20201025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1248839..1259966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7445_RS05885 (J7445_05885) lepB 1249996..1251015 (-) 1020 WP_209283321.1 signal peptidase I -
  J7445_RS05890 (J7445_05890) lepA 1251015..1252808 (-) 1794 WP_002226499.1 translation elongation factor 4 -
  J7445_RS05895 (J7445_05895) - 1252950..1253651 (-) 702 WP_209283322.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  J7445_RS05900 (J7445_05900) pilU 1253839..1254966 (+) 1128 WP_003742946.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J7445_RS12240 - 1255228..1255458 (-) 231 WP_029609610.1 tyrosine-type recombinase/integrase -
  J7445_RS05910 (J7445_05910) - 1255768..1256139 (-) 372 WP_244969530.1 integrase arm-type DNA-binding domain-containing protein -
  J7445_RS05950 (J7445_05950) pgsA 1257191..1257751 (-) 561 WP_019270169.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  J7445_RS05955 (J7445_05955) - 1257853..1258398 (-) 546 WP_019270168.1 hypothetical protein -
  J7445_RS05960 (J7445_05960) - 1258769..1259314 (-) 546 WP_019270167.1 FixH family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42142.36 Da        Isoelectric Point: 6.4185

>NTDB_id=553910 J7445_RS05900 WP_003742946.1 1253839..1254966(+) (pilU) [Neisseria sicca strain NS20201025]
MMTNPPSAKDELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKITPITDEPLTSEKCMEIAFSIMSTKQIEEFSSTNECNF
AISLPDTSRFRVNAMVQRGATALVFRSITSKIPNFDSLNLPPVLKDVVMKKRGLVIFVGGTGSGKSTSLAAMVDHRNENC
QDHIITIEDPIEFVHQHKKSIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCLATLHAN
NSNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPREGGKGRVAAVEILLNSPIISEMIQKGEIHGIKEMMKKSTA
MGMQTFDQALYELYERGDISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=553910 J7445_RS05900 WP_003742946.1 1253839..1254966(+) (pilU) [Neisseria sicca strain NS20201025]
ATGATGACCAACCCTCCCAGCGCAAAAGACGAATTGTTCACTTGGCTGCGCCATATGAACAAATACAAAGGCTCCGACCT
TTTTATCACTACCAATTTTCCGCCTGCTATGAAAGTGGACGGCAAAATTACGCCGATTACCGACGAGCCGCTGACCTCCG
AAAAATGTATGGAAATCGCTTTTTCGATTATGTCCACCAAACAAATCGAAGAGTTTTCCTCCACCAACGAATGCAACTTC
GCCATCAGCCTGCCCGACACCAGTCGCTTCCGCGTCAATGCCATGGTGCAGCGCGGCGCGACGGCGTTGGTGTTCCGCTC
CATTACCAGCAAAATCCCGAATTTCGACAGCCTCAATCTGCCGCCCGTCTTGAAAGACGTGGTGATGAAAAAACGCGGCC
TCGTGATTTTCGTCGGCGGTACCGGCTCGGGCAAATCGACCTCGCTCGCCGCGATGGTTGACCACCGCAACGAGAATTGC
CAAGACCACATCATCACCATCGAAGACCCGATTGAGTTCGTCCATCAACACAAAAAAAGCATCATTACCCAGCGCGAAGT
CGGCGTGGATACCGAAAACTGGTTTGCCGCGCTGAAAAACACCCTGCGTCAGGCGCCAGACGTCATCCTGATTGGTGAGA
TTCGCGACCGCGAAACCATGGACTACGCCTTGGCGTTCGCCGAAACAGGCCACCTCTGTCTCGCCACGCTGCACGCCAAC
AACTCCAACCAGGCGCTCGACCGTATCATCAACTTCTTCCCAGAAGAGCGCCGCACCCAGCTTTTGACCGACTTGTCGCT
TAACCTGCAAGCCTTCATCTCGCAACGCCTGATTCCCCGCGAAGGCGGCAAGGGCCGCGTAGCCGCAGTGGAAATCCTGC
TCAATTCGCCGATCATTTCCGAGATGATTCAAAAAGGCGAAATCCACGGCATCAAAGAAATGATGAAAAAATCGACCGCC
ATGGGCATGCAAACCTTCGACCAAGCGCTCTACGAGCTGTACGAACGCGGCGACATCAGCTTCCAAGACGCCATTAAAAA
TGCCGACTCCGCCCACGACCTGCGTTTGGATATCCAACTGCGCAGCCGCCGCGCCCAAAACGCCGGCCCAGATTTGGAAC
TGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1GN56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.523

93.867

0.549
  pilU Acinetobacter baylyi ADP1

58.017

91.467

0.531
  pilU Vibrio cholerae strain A1552

52.368

95.733

0.501
  pilT Acinetobacter nosocomialis M2

43.519

86.4

0.376
  pilT Acinetobacter baumannii D1279779

43.519

86.4

0.376
  pilT Acinetobacter baumannii strain A118

43.519

86.4

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.342

88.8

0.376
  pilT Legionella pneumophila strain Lp02

43.21

86.4

0.373
  pilT Legionella pneumophila strain ERS1305867

43.21

86.4

0.373
  pilT Pseudomonas aeruginosa PAK

42.073

87.467

0.368
  pilT Acinetobacter baylyi ADP1

41.768

87.467

0.365
  pilT Neisseria meningitidis 8013

40.299

89.333

0.36