Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6J77_RS04590 Genome accession   NZ_CP069535
Coordinates   1061457..1061879 (-) Length   140 a.a.
NCBI ID   WP_204110746.1    Uniprot ID   -
Organism   Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1044028..1061128 1061457..1061879 flank 329


Gene organization within MGE regions


Location: 1044028..1061879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J77_RS04515 (I6J77_04515) - 1044127..1045251 (+) 1125 WP_239309194.1 glycosyltransferase -
  I6J77_RS04520 (I6J77_04520) - 1045260..1046012 (+) 753 WP_204110734.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  I6J77_RS04525 (I6J77_04525) - 1046133..1047212 (-) 1080 WP_239309195.1 glycosyltransferase family 4 protein -
  I6J77_RS04530 (I6J77_04530) - 1047248..1048027 (-) 780 WP_204110735.1 glycosyltransferase family 2 protein -
  I6J77_RS04535 (I6J77_04535) - 1048046..1048753 (-) 708 WP_204110736.1 FkbM family methyltransferase -
  I6J77_RS04540 (I6J77_04540) - 1048791..1049789 (-) 999 WP_204110737.1 GDP-mannose 4,6-dehydratase -
  I6J77_RS04545 (I6J77_04545) - 1049795..1050898 (-) 1104 WP_204110738.1 glycosyltransferase family 1 protein -
  I6J77_RS04550 (I6J77_04550) - 1051152..1051928 (-) 777 WP_204110739.1 class I SAM-dependent methyltransferase -
  I6J77_RS04555 (I6J77_04555) - 1051918..1052358 (-) 441 WP_204110740.1 hypothetical protein -
  I6J77_RS04560 (I6J77_04560) - 1052901..1053524 (-) 624 WP_204110741.1 hypothetical protein -
  I6J77_RS04565 (I6J77_04565) - 1053595..1054920 (-) 1326 WP_204110742.1 flippase -
  I6J77_RS04570 (I6J77_04570) - 1054920..1055840 (-) 921 WP_204110743.1 GDP-L-fucose synthase -
  I6J77_RS04575 (I6J77_04575) gmd 1055845..1056954 (-) 1110 WP_204110744.1 GDP-mannose 4,6-dehydratase -
  I6J77_RS04580 (I6J77_04580) - 1057032..1059101 (-) 2070 WP_204111400.1 hypothetical protein -
  I6J77_RS04585 (I6J77_04585) - 1059404..1061275 (-) 1872 WP_204110745.1 lipopolysaccharide assembly protein LapB -
  I6J77_RS04590 (I6J77_04590) pilA 1061457..1061879 (-) 423 WP_204110746.1 pilin Machinery gene

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14902.10 Da        Isoelectric Point: 8.4716

>NTDB_id=535392 I6J77_RS04590 WP_204110746.1 1061457..1061879(-) (pilA) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
MKSMQKGFTLIELMIVVAIIAILAAIALPQYQNYVARSQATAGLADVTPGKTAYEEQVNNGIVGANSYTADLIGLQPTTE
RCAITTTAPTSGAANDAIKCALKGNPKVAGKYVEWNRNGSGLWTCKSDLDDKFRPKGCFN

Nucleotide


Download         Length: 423 bp        

>NTDB_id=535392 I6J77_RS04590 WP_204110746.1 1061457..1061879(-) (pilA) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
ATGAAGAGCATGCAGAAAGGTTTTACCCTGATCGAACTGATGATCGTGGTTGCGATCATCGCGATCTTGGCCGCCATCGC
GCTGCCGCAGTACCAGAACTATGTGGCTCGTTCGCAGGCGACTGCCGGCTTGGCTGACGTCACGCCCGGCAAGACCGCGT
ACGAAGAGCAGGTCAACAACGGTATCGTCGGCGCCAACTCGTACACGGCTGATCTGATCGGCCTTCAGCCCACCACCGAG
CGTTGCGCCATCACGACGACGGCTCCGACCTCTGGTGCCGCGAACGATGCGATCAAGTGCGCGCTGAAGGGTAACCCGAA
AGTCGCCGGCAAGTATGTCGAGTGGAACCGTAACGGCAGCGGCCTGTGGACGTGCAAGAGCGATCTGGACGACAAGTTCC
GCCCGAAGGGTTGCTTCAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.555

97.857

0.514
  pilA Pseudomonas aeruginosa PAK

46.207

100

0.479
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.826

98.571

0.471
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.366

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.559

100

0.414
  pilA Vibrio cholerae strain A1552

40.559

100

0.414
  pilA Vibrio cholerae C6706

40.559

100

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

35.714

100

0.393
  pilE Neisseria gonorrhoeae MS11

37.681

98.571

0.371
  pilA2 Legionella pneumophila strain ERS1305867

37.681

98.571

0.371
  pilA2 Legionella pneumophila str. Paris

38.235

97.143

0.371
  comP Acinetobacter baylyi ADP1

35.915

100

0.364