Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K8B71_RS27715 Genome accession   NZ_CP082823
Coordinates   5740374..5740841 (-) Length   155 a.a.
NCBI ID   WP_223119637.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5735374..5745841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B71_RS27695 (K8B71_27695) ampD 5736065..5736631 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8B71_RS27700 (K8B71_27700) - 5736782..5739037 (-) 2256 WP_003134886.1 DUF1631 domain-containing protein -
  K8B71_RS27705 (K8B71_27705) nadC 5739323..5740171 (+) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B71_RS27715 (K8B71_27715) pilA 5740374..5740841 (-) 468 WP_223119637.1 pilin Machinery gene
  K8B71_RS27720 (K8B71_27720) pilB 5741071..5742771 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8B71_RS27725 (K8B71_27725) pilC 5742775..5743995 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  K8B71_RS27730 (K8B71_27730) pilD 5743999..5744871 (+) 873 WP_003134888.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K8B71_RS27735 (K8B71_27735) coaE 5744868..5745479 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  K8B71_RS27740 (K8B71_27740) yacG 5745476..5745676 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15882.01 Da        Isoelectric Point: 6.1571

>NTDB_id=529941 K8B71_RS27715 WP_223119637.1 5740374..5740841(-) (pilA) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTTD

Nucleotide


Download         Length: 468 bp        

>NTDB_id=529941 K8B71_RS27715 WP_223119637.1 5740374..5740841(-) (pilA) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

99.355

0.465
  pilA Vibrio cholerae strain A1552

43.59

100

0.439
  pilA Vibrio cholerae C6706

43.59

100

0.439
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.439
  comP Acinetobacter baylyi ADP1

40.12

100

0.432
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.419
  pilA Acinetobacter baumannii strain A118

41.83

98.71

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.558

99.355

0.413
  pilA2 Legionella pneumophila str. Paris

40.909

99.355

0.406
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.4
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

97.419

0.374