Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K8B71_RS27730 Genome accession   NZ_CP082823
Coordinates   5743999..5744871 (+) Length   290 a.a.
NCBI ID   WP_003134888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5738999..5749871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B71_RS27705 (K8B71_27705) nadC 5739323..5740171 (+) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8B71_RS27715 (K8B71_27715) pilA 5740374..5740841 (-) 468 WP_223119637.1 pilin Machinery gene
  K8B71_RS27720 (K8B71_27720) pilB 5741071..5742771 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8B71_RS27725 (K8B71_27725) pilC 5742775..5743995 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  K8B71_RS27730 (K8B71_27730) pilD 5743999..5744871 (+) 873 WP_003134888.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K8B71_RS27735 (K8B71_27735) coaE 5744868..5745479 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  K8B71_RS27740 (K8B71_27740) yacG 5745476..5745676 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  K8B71_RS27745 (K8B71_27745) - 5745713..5745922 (-) 210 WP_003094660.1 hypothetical protein -
  K8B71_RS27750 (K8B71_27750) - 5746028..5746717 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  K8B71_RS27755 (K8B71_27755) - 5746714..5747184 (-) 471 WP_003094664.1 hypothetical protein -
  K8B71_RS27760 (K8B71_27760) - 5747181..5747606 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  K8B71_RS27765 (K8B71_27765) - 5747739..5748368 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  K8B71_RS27770 (K8B71_27770) - 5748365..5748814 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  K8B71_RS27775 (K8B71_27775) - 5748840..5749010 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31848.90 Da        Isoelectric Point: 7.7268

>NTDB_id=529944 K8B71_RS27730 WP_003134888.1 5743999..5744871(+) (pilD) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWHILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=529944 K8B71_RS27730 WP_003134888.1 5743999..5744871(+) (pilD) [Pseudomonas aeruginosa strain SCAID TST-2021 (7/157)]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCATATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472