Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JKK44_RS15500 Genome accession   NZ_CP068228
Coordinates   3437994..3439106 (-) Length   370 a.a.
NCBI ID   WP_025887558.1    Uniprot ID   A0A379YLK5
Organism   Shewanella algae strain G1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3432994..3444106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKK44_RS15480 (JKK44_15475) - 3434119..3434853 (-) 735 WP_226564522.1 tryptophan 2,3-dioxygenase family protein -
  JKK44_RS15485 (JKK44_15480) hemH 3435025..3436038 (-) 1014 WP_100145767.1 ferrochelatase -
  JKK44_RS15490 (JKK44_15485) - 3436073..3436669 (-) 597 WP_082014316.1 glutathione peroxidase -
  JKK44_RS15495 (JKK44_15490) - 3436727..3437836 (-) 1110 WP_039034903.1 hypothetical protein -
  JKK44_RS15500 (JKK44_15495) pilU 3437994..3439106 (-) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JKK44_RS15505 (JKK44_15500) pilT 3439116..3440153 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  JKK44_RS15510 (JKK44_15505) - 3440190..3440885 (+) 696 WP_115388995.1 YggS family pyridoxal phosphate-dependent enzyme -
  JKK44_RS15515 (JKK44_15510) proC 3441018..3441836 (+) 819 WP_115388996.1 pyrroline-5-carboxylate reductase -
  JKK44_RS15520 (JKK44_15515) - 3441860..3442411 (+) 552 WP_025887553.1 YggT family protein -
  JKK44_RS15525 (JKK44_15520) yggU 3442411..3442698 (+) 288 WP_037467122.1 DUF167 family protein YggU -
  JKK44_RS15530 (JKK44_15525) - 3442761..3443195 (+) 435 WP_025887551.1 DUF4426 domain-containing protein -
  JKK44_RS15535 (JKK44_15530) rdgB 3443415..3444017 (+) 603 WP_208165807.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=528276 JKK44_RS15500 WP_025887558.1 3437994..3439106(-) (pilU) [Shewanella algae strain G1]
MEVRPFLKAMVDRKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=528276 JKK44_RS15500 WP_025887558.1 3437994..3439106(-) (pilU) [Shewanella algae strain G1]
ATGGAAGTCAGACCTTTTCTCAAGGCTATGGTGGATCGCAAGGCCTCGGATCTCTTTATTACCGCGGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGACCCTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACACCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAAGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCTAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCACTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGCGGCCGGCGCGCCGCCATCGAAGTGCTGATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGACACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
CTGTTCAAGCTCTACTGTGAAGGAGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCACCTAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAATTCAGGTTTCATGGAGGGAGTTACCCTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YLK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.375

95.135

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362