Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JKK33_RS06190 Genome accession   NZ_CP068227
Coordinates   1385501..1386613 (+) Length   370 a.a.
NCBI ID   WP_025887558.1    Uniprot ID   A0A379YLK5
Organism   Shewanella algae strain A59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1380501..1391613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKK33_RS06155 (JKK33_06155) rdgB 1380590..1381192 (-) 603 WP_208125652.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JKK33_RS06160 (JKK33_06160) - 1381412..1381846 (-) 435 WP_025887551.1 DUF4426 domain-containing protein -
  JKK33_RS06165 (JKK33_06165) yggU 1381909..1382196 (-) 288 WP_037467122.1 DUF167 family protein YggU -
  JKK33_RS06170 (JKK33_06170) - 1382196..1382747 (-) 552 WP_044733985.1 YggT family protein -
  JKK33_RS06175 (JKK33_06175) proC 1382771..1383589 (-) 819 WP_144191065.1 pyrroline-5-carboxylate reductase -
  JKK33_RS06180 (JKK33_06180) - 1383722..1384417 (-) 696 WP_208125653.1 YggS family pyridoxal phosphate-dependent enzyme -
  JKK33_RS06185 (JKK33_06185) pilT 1384454..1385491 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  JKK33_RS06190 (JKK33_06190) pilU 1385501..1386613 (+) 1113 WP_025887558.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JKK33_RS06195 (JKK33_06195) - 1386738..1387847 (+) 1110 WP_052678495.1 hypothetical protein -
  JKK33_RS06200 (JKK33_06200) - 1387887..1388468 (+) 582 WP_081737044.1 glutathione peroxidase -
  JKK33_RS06205 (JKK33_06205) hemH 1388503..1389516 (+) 1014 WP_025009459.1 ferrochelatase -
  JKK33_RS06210 (JKK33_06210) - 1389692..1390426 (+) 735 WP_071237900.1 tryptophan 2,3-dioxygenase family protein -
  JKK33_RS06215 (JKK33_06215) - 1390437..1391567 (+) 1131 WP_208125654.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=528241 JKK33_RS06190 WP_025887558.1 1385501..1386613(+) (pilU) [Shewanella algae strain A59]
MEVRPFLKAMVDRKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=528241 JKK33_RS06190 WP_025887558.1 1385501..1386613(+) (pilU) [Shewanella algae strain A59]
ATGGAAGTCAGACCTTTTCTCAAAGCCATGGTGGATCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGCTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAAGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGTATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTAGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCCAATGCCAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGCGGCCGGCGCGCCGCCATCGAAGTGCTGATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGACACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
TTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCGCCAAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGTAACTCAGGTTTCATGGAGGGAGTCACCCTGGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379YLK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.375

95.135

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter baumannii D1279779

40.351

92.432

0.373
  pilT Acinetobacter baumannii strain A118

40.351

92.432

0.373
  pilT Acinetobacter nosocomialis M2

40.351

92.432

0.373
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362