Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6I53_RS01725 Genome accession   NZ_CP068176
Coordinates   254734..255852 (+) Length   372 a.a.
NCBI ID   WP_004995983.1    Uniprot ID   A0A7T9Z738
Organism   Acinetobacter ursingii strain FDAARGOS_1096     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 249734..260852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I53_RS01705 (I6I53_01710) - 250971..251762 (+) 792 WP_004995989.1 transglutaminase family protein -
  I6I53_RS01710 (I6I53_01715) - 251863..252708 (-) 846 WP_004995986.1 undecaprenyl-diphosphate phosphatase -
  I6I53_RS01715 (I6I53_01720) - 252859..253545 (-) 687 WP_004995984.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6I53_RS01720 (I6I53_01725) pilT 253669..254706 (+) 1038 WP_004990994.1 type IV pilus twitching motility protein PilT Machinery gene
  I6I53_RS01725 (I6I53_01730) pilU 254734..255852 (+) 1119 WP_004995983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6I53_RS01730 (I6I53_01735) fur 255926..256366 (-) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  I6I53_RS01735 (I6I53_01740) - 256478..256876 (+) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  I6I53_RS01740 (I6I53_01745) - 256920..257240 (-) 321 WP_004995981.1 RnfH family protein -
  I6I53_RS01745 (I6I53_01750) - 257237..258322 (-) 1086 WP_004995978.1 hypothetical protein -
  I6I53_RS01750 (I6I53_01755) - 258485..258934 (-) 450 WP_004991008.1 bacteriohemerythrin -
  I6I53_RS01755 (I6I53_01760) - 259270..260118 (-) 849 WP_035254157.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41836.03 Da        Isoelectric Point: 6.0604

>NTDB_id=527803 I6I53_RS01725 WP_004995983.1 254734..255852(+) (pilU) [Acinetobacter ursingii strain FDAARGOS_1096]
MDFNDLLNLMIEQQASDLFVTAGVEPSMKINGQIVPVSKTKLNAEVVGQLIQSIMSDKQRKEFDESRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETIIPTMDELRLPPILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITVE
DPIEFIHEHAGCIVTQREVGIDTDSFDIALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFESDRHNQLYMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLSIKLSEEGAEQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=527803 I6I53_RS01725 WP_004995983.1 254734..255852(+) (pilU) [Acinetobacter ursingii strain FDAARGOS_1096]
ATGGATTTTAATGATCTTCTCAATCTGATGATTGAACAACAAGCATCAGACCTGTTTGTCACCGCAGGTGTGGAACCTTC
GATGAAAATTAATGGTCAGATTGTCCCAGTGTCTAAGACTAAGCTGAATGCCGAGGTCGTTGGACAACTGATTCAGTCCA
TTATGTCTGATAAGCAACGCAAGGAATTTGACGAGAGCAGAGAGTGTAACTTTGCGATTGTAAATCGGGAAAAAACCGCA
CGTTTTCGTGTCAGTGCATTTCAGCAGCGTGATATGCCAGGTATGGTGCTGCGTCGCATTGAAACGATTATCCCTACAAT
GGATGAATTACGCTTACCGCCCATTCTTAAAGATCTGGCCATGACCAAACGTGGCATTATTATCTTTGTTGGGGCAACAG
GTACGGGTAAATCGACTTCATTGGCATCCATGATTGGTTATCGAAATCAGAATTCCAAAGGTCATATCATCACGGTTGAA
GATCCAATCGAGTTCATTCATGAACACGCTGGTTGTATTGTGACTCAACGTGAAGTCGGTATTGATACTGACTCTTTCGA
TATAGCCCTCAAAAACACACTACGACAAGCCCCTGATGTGATTTTGATTGGTGAGATTCGTTCACGTGAGGTCATGGACT
ATGCCATTGCCTTTGCTGAAACGGGACATCTGGTCTTAGCGACATTACACGCCAACAACGCCAACCAAGCATTGGATCGT
ATTATTCATTTCTTTGAAAGTGATCGCCATAATCAGCTCTATATGGACCTCTCGCTCAATCTAAAAGCCATGGTGGCACA
GCAATTGATTCCAACACCAGATGGCAATTCACGTCGTGCTGCGATTGAAATTCTGATCAATACACCATTACTTGCTGACT
ATATTCGTAAAGGTGAAATTCATGAAATCAAAGATTTAATGAAACGTTCACGTGAACTCGGCATGCAAACCTTTGATCAG
GCTTTATTTGATCTTTATAAAGCAGGTCAAATTACCTATAAAGATGCCTTAAAACATGCTGACTCGCCAAACGATCTACG
CCTCAGCATCAAACTTTCAGAGGAAGGTGCAGAACAACTATTAAATGCCAGTCGCAACATTACTTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

77.249

100

0.785
  pilU Pseudomonas stutzeri DSM 10701

69.542

99.731

0.694
  pilU Vibrio cholerae strain A1552

55.556

94.355

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.484

97.849

0.406
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter baylyi ADP1

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36