Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6I53_RS01720 Genome accession   NZ_CP068176
Coordinates   253669..254706 (+) Length   345 a.a.
NCBI ID   WP_004990994.1    Uniprot ID   A0A2N6VBT8
Organism   Acinetobacter ursingii strain FDAARGOS_1096     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 248669..259706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I53_RS01705 (I6I53_01710) - 250971..251762 (+) 792 WP_004995989.1 transglutaminase family protein -
  I6I53_RS01710 (I6I53_01715) - 251863..252708 (-) 846 WP_004995986.1 undecaprenyl-diphosphate phosphatase -
  I6I53_RS01715 (I6I53_01720) - 252859..253545 (-) 687 WP_004995984.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6I53_RS01720 (I6I53_01725) pilT 253669..254706 (+) 1038 WP_004990994.1 type IV pilus twitching motility protein PilT Machinery gene
  I6I53_RS01725 (I6I53_01730) pilU 254734..255852 (+) 1119 WP_004995983.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6I53_RS01730 (I6I53_01735) fur 255926..256366 (-) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  I6I53_RS01735 (I6I53_01740) - 256478..256876 (+) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  I6I53_RS01740 (I6I53_01745) - 256920..257240 (-) 321 WP_004995981.1 RnfH family protein -
  I6I53_RS01745 (I6I53_01750) - 257237..258322 (-) 1086 WP_004995978.1 hypothetical protein -
  I6I53_RS01750 (I6I53_01755) - 258485..258934 (-) 450 WP_004991008.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38313.90 Da        Isoelectric Point: 6.5052

>NTDB_id=527802 I6I53_RS01720 WP_004990994.1 253669..254706(+) (pilT) [Acinetobacter ursingii strain FDAARGOS_1096]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQTARTAAKSPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=527802 I6I53_RS01720 WP_004990994.1 253669..254706(+) (pilT) [Acinetobacter ursingii strain FDAARGOS_1096]
ATGGATATCACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCATCAGATTTACATCTTTCCGCAGGAATGCCACCGAT
GATTCGTGTCGATGGTGAGGTTCGTCGTATTAATCTACCTGCACTGGAACACAAAGAAGTTCATCGTCTTGTTTATGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAACAGCTAGAAACTGACTTTTCCTTTGAAGTTCCAAACATTGCGCGT
TTTCGTGTCAATGCCTTTAACCAAAATCGTGGTGCAGGTGCTGTGTTCCGTACCATTCCGTCTAAAGTTCTCACCATGGA
TGATTTGGGTTTAGGACAAATCTTTAAGGATATTTGTGATTATCCACGTGGTATTGTACTGGTTACAGGCCCAACGGGTT
CGGGTAAATCAACCACGCTTGCCGCGATGCTAGATTATATTAATGACAATCGCTATGACCATATTTTAACCGTAGAAGAT
CCGATCGAATTTGTACATCAATCCAAAAAGTGCCTGATTAACCAACGTGAAGTACATCGTGATACTCATGGCTTTAATGA
AGCACTACGTTCAGCACTACGTGAAGACCCTGATATTATCCTTGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CTTTGACCGCCGCAGAAACGGGTCACTTGGTATTTGGTACATTGCATACCACATCTGCTGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGCTATCTGAATCTTTACAAGCCGTTATTTCTCAAAC
ATTATTGAAGAAAAATGGCGGTGGCCGTGTGGCAGCGCATGAGATCATGATCGGGATACCTGCGATCCGTAACTTAATCC
GTGAAAATAAAGTCGCGCAAATGTACTCTGCTATTCAGACAGGTGCTAACTATGGAATGACCACGCTAGATCAAAGCCTC
AAAACATTGGTTTCAAAAGGCGTGATCAGTCCACAAACTGCGCGAACAGCTGCCAAATCACCCGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N6VBT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.762

98.841

0.403
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397