Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   JJN14_RS00610 Genome accession   NZ_CP067992
Coordinates   151157..152098 (+) Length   313 a.a.
NCBI ID   WP_201058644.1    Uniprot ID   -
Organism   Streptococcus mitis strain S022-V7-A3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146157..157098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN14_RS00595 (JJN14_00595) pbp1b 146588..149038 (+) 2451 WP_070479589.1 penicillin-binding protein PBP1B -
  JJN14_RS00600 (JJN14_00600) - 149507..150565 (+) 1059 WP_201058643.1 zinc-dependent alcohol dehydrogenase family protein -
  JJN14_RS00605 (JJN14_00605) - 150715..151080 (+) 366 WP_033688032.1 DUF1033 family protein -
  JJN14_RS00610 (JJN14_00610) comGA/cglA/cilD 151157..152098 (+) 942 WP_201058644.1 competence type IV pilus ATPase ComGA Machinery gene
  JJN14_RS00615 (JJN14_00615) comGB/cglB 152046..153062 (+) 1017 WP_201059119.1 competence type IV pilus assembly protein ComGB Machinery gene
  JJN14_RS00620 (JJN14_00620) comGC/cglC 153064..153390 (+) 327 WP_201058645.1 comG operon protein ComGC Machinery gene
  JJN14_RS00625 (JJN14_00625) comGD/cglD 153383..153787 (+) 405 WP_265342534.1 competence type IV pilus minor pilin ComGD Machinery gene
  JJN14_RS00630 (JJN14_00630) comGE/cglE 153750..154052 (+) 303 WP_255262223.1 competence type IV pilus minor pilin ComGE Machinery gene
  JJN14_RS00635 (JJN14_00635) comGF/cglF 154015..154476 (+) 462 WP_201058647.1 competence type IV pilus minor pilin ComGF Machinery gene
  JJN14_RS00640 (JJN14_00640) comGG/cglG 154454..154867 (+) 414 WP_201058648.1 competence type IV pilus minor pilin ComGG Machinery gene
  JJN14_RS00645 (JJN14_00645) - 154900..155487 (+) 588 Protein_122 class I SAM-dependent methyltransferase -
  JJN14_RS00650 (JJN14_00650) comYH 155548..156501 (+) 954 WP_201058649.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.40 Da        Isoelectric Point: 5.6169

>NTDB_id=526123 JJN14_RS00610 WP_201058644.1 151157..152098(+) (comGA/cglA/cilD) [Streptococcus mitis strain S022-V7-A3]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHNEEQDLHFWFQDIEELGNQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQVDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=526123 JJN14_RS00610 WP_201058644.1 151157..152098(+) (comGA/cglA/cilD) [Streptococcus mitis strain S022-V7-A3]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAGGATATTTATTTTGTTCCTAAGTT
AGACGCTTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCGGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGTCAACTTGGTTCTTGTGATTATGAA
TATGACCAGAAGATGGCGTCCTTACGTTTATCTACTGTAGGCGACTATCGAGGGCATGAGAGCTTAGTGATTCGCTTGTT
GCATAATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATCGAAGAACTGGGCAATCAGTACAGGCAACGTGGTCTCT
ATCTTTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCCTGATGCACGAATTAGCTAAATCTCTCTTTAAAGGACAGCAA
GTCATGTCCATTGAAGATCCAGTCGAGATTAAGCAGGACGATATGCTCCAGTTGCAGTTGAATGAGGCGATAGGGCTTAC
CTATGAAAATCTCATCAAACTTTCCCTACGGCATCGTCCTGATCTCCTGATTATCGGAGAAATTAGGGACAGCGAAACGG
CACGTGCAGTGGTTAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCCTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCCAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGGTTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.764

100

0.978
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

99.042

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

43.019

84.665

0.364