Detailed information NTG0526124

Bioinformatically predicted

Overview

• Name: comGB/cglB
• Type: Machinery gene
• Locus tag: JJN14_RS00615
• Genome accession: NZ_CP067992
• Coordinates: 152046..153062 (+)
• Length: 338 a.a.
• NCBI ID: WP_201059119.1
• Uniprot ID: -
• Organism: Streptococcus mitis strain S022-V7-A3
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 147046..158062

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JJN14_RS00600 (JJN14_00600)	-	149507..150565 (+)	1059	WP_201058643.1	zinc-dependent alcohol dehydrogenase family protein	-
JJN14_RS00605 (JJN14_00605)	-	150715..151080 (+)	366	WP_033688032.1	DUF1033 family protein	-
JJN14_RS00610 (JJN14_00610)	comGA/cglA/cilD	151157..152098 (+)	942	WP_201058644.1	competence type IV pilus ATPase ComGA	Machinery gene
JJN14_RS00615 (JJN14_00615)	comGB/cglB	152046..153062 (+)	1017	WP_201059119.1	competence type IV pilus assembly protein ComGB	Machinery gene
JJN14_RS00620 (JJN14_00620)	comGC/cglC	153064..153390 (+)	327	WP_201058645.1	comG operon protein ComGC	Machinery gene
JJN14_RS00625 (JJN14_00625)	comGD/cglD	153383..153787 (+)	405	WP_265342534.1	competence type IV pilus minor pilin ComGD	Machinery gene
JJN14_RS00630 (JJN14_00630)	comGE/cglE	153750..154052 (+)	303	WP_255262223.1	competence type IV pilus minor pilin ComGE	Machinery gene
JJN14_RS00635 (JJN14_00635)	comGF/cglF	154015..154476 (+)	462	WP_201058647.1	competence type IV pilus minor pilin ComGF	Machinery gene
JJN14_RS00640 (JJN14_00640)	comGG/cglG	154454..154867 (+)	414	WP_201058648.1	competence type IV pilus minor pilin ComGG	Machinery gene
JJN14_RS00645 (JJN14_00645)	-	154900..155487 (+)	588	Protein_122	class I SAM-dependent methyltransferase	-
JJN14_RS00650 (JJN14_00650)	comYH	155548..156501 (+)	954	WP_201058649.1	class I SAM-dependent methyltransferase	Machinery gene
JJN14_RS00655 (JJN14_00655)	-	156551..157741 (+)	1191	WP_201058650.1	acetate kinase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 38498.49 Da
• Isoelectric Point: 9.7176

>NTDB_id=526124 JJN14_RS00615 WP_201059119.1 152046..153062(+) (comGB/cglB) [Streptococcus mitis strain S022-V7-A3]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILALLALTFYKRSSKMRVFSILARLPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSRLFKEI
GQNLAQALQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide

• Length: 1017 bp

>NTDB_id=526124 JJN14_RS00615 WP_201059119.1 152046..153062(+) (comGB/cglB) [Streptococcus mitis strain S022-V7-A3]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGATAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGTTTTTCAAGTGCCATT
GTCACTCAGTTATCTCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGATTTCTTCTCTTAATCATGCTGG
GGTTACGTAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATTATTGGTAATCTGCCACAAATTTTT
CTAGGAATGGTAGGTTTTGTTTCCATACTTGCCCTTTTAGCGCTAACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCACGCCTTCCTTTCTTTGGAATATTTGTTCAGACCTATTTGACAGCCTATTATGCGCGTGAGTGGGGGA
ATATGATTTCGCAGGGAATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAAGGTTCTCGGCTCTTTAAAGAAATC
GGTCAAAATCTGGCTCAAGCCCTACAAAATGGTCAGGAATTTTCTCAGACTATAGGAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGCGAGTTGGAAATCTATGCGGAAAAGACTTGGG
AAGCCTTTTTTACCCGAGTCAATCGCACCATGAATCTGGTGCAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTACGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (24-141)

• Type II secretion system (T2SS), protein F (T2SSF) (209-329)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB/cglB	Streptococcus mitis NCTC 12261	96.746	100	0.967
comGB/cglB	Streptococcus mitis SK321	96.45	100	0.965
comGB/cglB	Streptococcus pneumoniae Rx1	95.562	100	0.956
comGB/cglB	Streptococcus pneumoniae D39	95.562	100	0.956
comGB/cglB	Streptococcus pneumoniae R6	95.562	100	0.956
comGB/cglB	Streptococcus pneumoniae TIGR4	95.562	100	0.956
comYB	Streptococcus gordonii str. Challis substr. CH1	73.214	99.408	0.728
comYB	Streptococcus mutans UA140	57.911	93.491	0.541
comYB	Streptococcus mutans UA159	57.911	93.491	0.541
comGB	Lactococcus lactis subsp. cremoris KW2	51.497	98.817	0.509