Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   JJN14_RS00630 Genome accession   NZ_CP067992
Coordinates   153750..154052 (+) Length   100 a.a.
NCBI ID   WP_255262223.1    Uniprot ID   -
Organism   Streptococcus mitis strain S022-V7-A3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 148750..159052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN14_RS00600 (JJN14_00600) - 149507..150565 (+) 1059 WP_201058643.1 zinc-dependent alcohol dehydrogenase family protein -
  JJN14_RS00605 (JJN14_00605) - 150715..151080 (+) 366 WP_033688032.1 DUF1033 family protein -
  JJN14_RS00610 (JJN14_00610) comGA/cglA/cilD 151157..152098 (+) 942 WP_201058644.1 competence type IV pilus ATPase ComGA Machinery gene
  JJN14_RS00615 (JJN14_00615) comGB/cglB 152046..153062 (+) 1017 WP_201059119.1 competence type IV pilus assembly protein ComGB Machinery gene
  JJN14_RS00620 (JJN14_00620) comGC/cglC 153064..153390 (+) 327 WP_201058645.1 comG operon protein ComGC Machinery gene
  JJN14_RS00625 (JJN14_00625) comGD/cglD 153383..153787 (+) 405 WP_265342534.1 competence type IV pilus minor pilin ComGD Machinery gene
  JJN14_RS00630 (JJN14_00630) comGE/cglE 153750..154052 (+) 303 WP_255262223.1 competence type IV pilus minor pilin ComGE Machinery gene
  JJN14_RS00635 (JJN14_00635) comGF/cglF 154015..154476 (+) 462 WP_201058647.1 competence type IV pilus minor pilin ComGF Machinery gene
  JJN14_RS00640 (JJN14_00640) comGG/cglG 154454..154867 (+) 414 WP_201058648.1 competence type IV pilus minor pilin ComGG Machinery gene
  JJN14_RS00645 (JJN14_00645) - 154900..155487 (+) 588 Protein_122 class I SAM-dependent methyltransferase -
  JJN14_RS00650 (JJN14_00650) comYH 155548..156501 (+) 954 WP_201058649.1 class I SAM-dependent methyltransferase Machinery gene
  JJN14_RS00655 (JJN14_00655) - 156551..157741 (+) 1191 WP_201058650.1 acetate kinase -
  JJN14_RS00660 (JJN14_00660) rnpA 157890..158261 (+) 372 WP_201058651.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11163.21 Da        Isoelectric Point: 10.0522

>NTDB_id=526127 JJN14_RS00630 WP_255262223.1 153750..154052(+) (comGE/cglE) [Streptococcus mitis strain S022-V7-A3]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEILRVAKMALQTGQNQVKINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=526127 JJN14_RS00630 WP_255262223.1 153750..154052(+) (comGE/cglE) [Streptococcus mitis strain S022-V7-A3]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCCGTAGTTGCTCTAGCTATCTT
TGCCAGCATTGCGACCCTCCTTTTGGGCCAAATTCAGAAAAATAGGCAAGAAGAAGCAAAAATCTTGCAGAAAGAAGAGA
TCTTGCGTGTAGCTAAGATGGCTTTGCAGACAGGTCAAAATCAGGTAAAGATAAACGGAGTTGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTTTATCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis SK321

100

100

1
  comGE/cglE Streptococcus mitis NCTC 12261

99

100

0.99
  comGE/cglE Streptococcus pneumoniae Rx1

98

100

0.98
  comGE/cglE Streptococcus pneumoniae D39

98

100

0.98
  comGE/cglE Streptococcus pneumoniae R6

98

100

0.98
  comGE/cglE Streptococcus pneumoniae TIGR4

98

100

0.98
  comYE Streptococcus mutans UA140

44.444

90

0.4
  comYE Streptococcus mutans UA159

44.444

90

0.4