Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JHW38_RS25700 Genome accession   NZ_CP067395
Coordinates   2698233..2698655 (+) Length   140 a.a.
NCBI ID   WP_207525978.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2693233..2703655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS11185 (JHW38_11185) pilR 2694920..2696344 (+) 1425 WP_207525976.1 sigma-54 dependent transcriptional regulator Regulator
  JHW38_RS11190 (JHW38_11190) pilA 2696969..2697391 (+) 423 WP_207525977.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  JHW38_RS25700 (JHW38_11195) pilA2 2698233..2698655 (+) 423 WP_207525978.1 pilin Machinery gene
  JHW38_RS25705 - 2698729..2699220 (+) 492 WP_278249813.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JHW38_RS11205 (JHW38_11205) - 2699343..2701235 (+) 1893 WP_207525979.1 hypothetical protein -
  JHW38_RS11210 (JHW38_11210) - 2701237..2703219 (+) 1983 WP_207525980.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14435.63 Da        Isoelectric Point: 8.4832

>NTDB_id=525860 JHW38_RS25700 WP_207525978.1 2698233..2698655(+) (pilA2) [Lysobacter enzymogenes strain CX03]
MKKQQGFTLIELMIVIAILGILIAIALPAYQDYTIRTKNAECINVAAAAKLAVSETAQDRGSLGAITSQAITGYSFAASS
YCASVTIAAGGEITATTQATGAGTLAVFKLTPSSNTGRIDWACTESAGAKKSQLPAECRK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=525860 JHW38_RS25700 WP_207525978.1 2698233..2698655(+) (pilA2) [Lysobacter enzymogenes strain CX03]
ATGAAGAAGCAGCAAGGCTTTACCCTGATCGAACTGATGATCGTCATCGCGATCCTGGGCATCCTGATCGCCATCGCTCT
GCCGGCCTATCAGGACTACACCATCCGCACCAAGAACGCTGAGTGCATCAACGTCGCCGCCGCCGCCAAGCTGGCCGTGT
CGGAAACCGCTCAGGACCGCGGCTCGCTGGGCGCGATCACCTCGCAGGCGATCACCGGCTACAGCTTCGCTGCCAGCAGC
TACTGCGCCAGCGTGACCATCGCCGCCGGCGGCGAAATCACCGCCACCACCCAGGCCACCGGCGCTGGCACCCTCGCGGT
CTTCAAGCTGACCCCGAGCTCCAACACCGGCCGCATCGACTGGGCTTGCACCGAGAGCGCCGGCGCCAAGAAGTCGCAGC
TGCCGGCCGAGTGCCGCAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.773

100

0.521
  pilA2 Legionella pneumophila strain ERS1305867

51.064

100

0.514
  pilA Ralstonia pseudosolanacearum GMI1000

40.964

100

0.486
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.623

100

0.45
  comP Acinetobacter baylyi ADP1

40.789

100

0.443
  pilE Neisseria gonorrhoeae strain FA1090

37.419

100

0.414
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.4
  pilE Neisseria gonorrhoeae MS11

35.897

100

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.13

98.571

0.386
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.686

97.857

0.379