Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JHW38_RS11190 Genome accession   NZ_CP067395
Coordinates   2696969..2697391 (+) Length   140 a.a.
NCBI ID   WP_207525977.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2691969..2702391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS11180 (JHW38_11180) - 2693197..2694822 (+) 1626 WP_207525975.1 ATP-binding protein -
  JHW38_RS11185 (JHW38_11185) pilR 2694920..2696344 (+) 1425 WP_207525976.1 sigma-54 dependent transcriptional regulator Regulator
  JHW38_RS11190 (JHW38_11190) pilA 2696969..2697391 (+) 423 WP_207525977.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  JHW38_RS25700 (JHW38_11195) pilA2 2698233..2698655 (+) 423 WP_207525978.1 pilin Machinery gene
  JHW38_RS25705 - 2698729..2699220 (+) 492 WP_278249813.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JHW38_RS11205 (JHW38_11205) - 2699343..2701235 (+) 1893 WP_207525979.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14757.99 Da        Isoelectric Point: 8.1029

>NTDB_id=525850 JHW38_RS11190 WP_207525977.1 2696969..2697391(+) (pilA) [Lysobacter enzymogenes strain CX03]
MNKQQGFTLIELMIVIAILGILIAIALPAYQDYTIRTKNAECLNVAAAAKLAVSETAQDRGSLGAITAQSITGYSFSASE
YCADVTIGVKGKITATTRATGGTPSAVFELTPSADLGRIDWVCKETNKARKSQLPAECRG

Nucleotide


Download         Length: 423 bp        

>NTDB_id=525850 JHW38_RS11190 WP_207525977.1 2696969..2697391(+) (pilA) [Lysobacter enzymogenes strain CX03]
ATGAACAAGCAGCAAGGCTTCACCCTGATCGAACTGATGATCGTGATCGCGATCCTCGGCATCCTGATCGCGATCGCGTT
GCCGGCGTATCAGGACTACACCATCCGTACCAAGAACGCCGAGTGCTTGAACGTTGCCGCCGCGGCGAAGCTGGCGGTTT
CCGAGACCGCCCAGGATCGCGGTTCGCTCGGTGCGATCACCGCGCAGTCCATCACCGGCTACAGCTTCAGCGCCAGCGAG
TACTGCGCCGACGTGACCATCGGCGTCAAGGGCAAAATTACCGCCACCACCCGCGCCACCGGCGGCACCCCTTCGGCTGT
CTTCGAACTGACCCCCTCCGCCGATCTCGGCCGTATCGACTGGGTCTGCAAGGAAACCAACAAGGCCCGCAAGTCGCAGC
TGCCGGCCGAGTGCAGGGGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

43.114

100

0.514
  pilA2 Legionella pneumophila str. Paris

49.275

98.571

0.486
  comP Acinetobacter baylyi ADP1

43.506

100

0.479
  pilA2 Legionella pneumophila strain ERS1305867

47.826

98.571

0.471
  pilE Neisseria gonorrhoeae strain FA1090

35.404

100

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.571

100

0.386
  pilE Neisseria gonorrhoeae MS11

33.333

100

0.371