Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JGR64_RS10970 Genome accession   NZ_CP067112
Coordinates   2387659..2388093 (+) Length   144 a.a.
NCBI ID   WP_199373413.1    Uniprot ID   -
Organism   Luteimonas sp. MC1572     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382659..2393093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR64_RS10935 (JGR64_10935) - 2382761..2383492 (-) 732 WP_199373406.1 methyltransferase domain-containing protein -
  JGR64_RS10940 (JGR64_10940) - 2383489..2384148 (-) 660 WP_199373407.1 class I SAM-dependent methyltransferase -
  JGR64_RS10945 (JGR64_10945) - 2384145..2384492 (-) 348 WP_199373408.1 DUF2304 domain-containing protein -
  JGR64_RS10950 (JGR64_10950) - 2384489..2385229 (-) 741 WP_199373409.1 glycosyltransferase family 2 protein -
  JGR64_RS10955 (JGR64_10955) - 2385240..2386049 (-) 810 WP_199373410.1 glycosyltransferase family A protein -
  JGR64_RS10960 (JGR64_10960) - 2386049..2386432 (-) 384 WP_199373411.1 hypothetical protein -
  JGR64_RS10965 (JGR64_10965) - 2386586..2387278 (+) 693 WP_199373412.1 hypothetical protein -
  JGR64_RS10970 (JGR64_10970) pilA2 2387659..2388093 (+) 435 WP_199373413.1 pilin Machinery gene
  JGR64_RS10975 (JGR64_10975) - 2388198..2390111 (+) 1914 WP_199373414.1 hypothetical protein -
  JGR64_RS10980 (JGR64_10980) pilB 2390230..2391954 (+) 1725 WP_199373415.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14670.03 Da        Isoelectric Point: 7.8231

>NTDB_id=525101 JGR64_RS10970 WP_199373413.1 2387659..2388093(+) (pilA2) [Luteimonas sp. MC1572]
MKKIQKGFTLIELMIVIAILGILVAIALPAYQDYSIRARVSEGVAQAAPAKLAVAETASARGILATAVTDASAAGFSFAA
NATDYVASIGVASGVITVTTKATGATVQPILTLSPSQAAVDQPVNWVCAFTAGEARHVPAECRP

Nucleotide


Download         Length: 435 bp        

>NTDB_id=525101 JGR64_RS10970 WP_199373413.1 2387659..2388093(+) (pilA2) [Luteimonas sp. MC1572]
ATGAAGAAGATCCAGAAGGGCTTTACCCTCATCGAACTGATGATCGTGATCGCGATCCTCGGCATCCTCGTTGCCATCGC
GCTGCCGGCGTACCAGGACTACAGCATCCGCGCCCGCGTTTCGGAAGGCGTTGCCCAGGCCGCTCCGGCGAAGCTTGCTG
TTGCCGAAACTGCCTCCGCACGCGGCATCCTTGCAACTGCTGTGACCGACGCTTCGGCTGCCGGTTTCAGCTTTGCCGCT
AACGCAACCGACTACGTGGCCAGCATTGGCGTCGCGAGCGGCGTTATCACCGTGACCACGAAGGCGACGGGCGCCACCGT
GCAGCCGATTCTGACCCTCAGCCCCAGCCAGGCAGCCGTCGACCAGCCGGTCAACTGGGTCTGCGCCTTCACCGCTGGCG
AGGCGCGTCACGTGCCGGCCGAGTGCCGCCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.448

99.306

0.521
  pilA2 Legionella pneumophila strain ERS1305867

51.748

99.306

0.514
  comP Acinetobacter baylyi ADP1

48.026

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

42.262

100

0.493
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.602

100

0.472
  pilE Neisseria gonorrhoeae strain FA1090

40.373

100

0.451
  pilA/pilA1 Eikenella corrodens VA1

41.176

100

0.437
  pilE Neisseria gonorrhoeae MS11

37.805

100

0.431
  pilA Haemophilus influenzae Rd KW20

37.931

100

0.382
  pilA Haemophilus influenzae 86-028NP

36.552

100

0.368
  pilA Vibrio parahaemolyticus RIMD 2210633

38.235

94.444

0.361