Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JGR64_RS10980 Genome accession   NZ_CP067112
Coordinates   2390230..2391954 (+) Length   574 a.a.
NCBI ID   WP_199373415.1    Uniprot ID   -
Organism   Luteimonas sp. MC1572     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2385230..2396954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR64_RS10955 (JGR64_10955) - 2385240..2386049 (-) 810 WP_199373410.1 glycosyltransferase family A protein -
  JGR64_RS10960 (JGR64_10960) - 2386049..2386432 (-) 384 WP_199373411.1 hypothetical protein -
  JGR64_RS10965 (JGR64_10965) - 2386586..2387278 (+) 693 WP_199373412.1 hypothetical protein -
  JGR64_RS10970 (JGR64_10970) pilA2 2387659..2388093 (+) 435 WP_199373413.1 pilin Machinery gene
  JGR64_RS10975 (JGR64_10975) - 2388198..2390111 (+) 1914 WP_199373414.1 hypothetical protein -
  JGR64_RS10980 (JGR64_10980) pilB 2390230..2391954 (+) 1725 WP_199373415.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JGR64_RS10985 (JGR64_10985) pilC 2392248..2393402 (+) 1155 WP_233348192.1 type II secretion system F family protein Machinery gene
  JGR64_RS10990 (JGR64_10990) - 2393407..2394267 (+) 861 WP_199373417.1 A24 family peptidase -
  JGR64_RS10995 (JGR64_10995) coaE 2394312..2394935 (+) 624 WP_199373418.1 dephospho-CoA kinase -
  JGR64_RS11000 (JGR64_11000) - 2394940..2395398 (-) 459 WP_199373419.1 hypothetical protein -
  JGR64_RS11005 (JGR64_11005) - 2395395..2396945 (-) 1551 WP_200649742.1 DUF2235 domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62652.90 Da        Isoelectric Point: 5.4611

>NTDB_id=525102 JGR64_RS10980 WP_199373415.1 2390230..2391954(+) (pilB) [Luteimonas sp. MC1572]
MSAVQTSNLAGITGIARRLVLDGALDEATARDSLDRATAERKPIASYLREAKLVTSAQMAAANSIEFGMPVFDPSVMDGT
QSAIKLVTDELLAKHNVLPLFKRGGRLFVGVADPTNTHALDEVKFHTNLTVEPILVDEERIRRTIELWQESSDTFGDTLD
DSEGLEKLDISGGEEDGTDDGVSAKGDDTPVVKFINKVLIDAIKKGASDIHFEPYETDYRVRLRIDGILKQVAKMPVKLN
QRITARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGVEKLGYEPEQQKLFLE
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSKCKQPQELPDNAL
LAEGFTADEIHEGFTIYEAVGCDECTGGYKGRLGVYQVLPMSEEIQTIVLQGGNAIQIAEVAKAAGVKDLRQSALLKVKN
GLTTLAEINRITVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=525102 JGR64_RS10980 WP_199373415.1 2390230..2391954(+) (pilB) [Luteimonas sp. MC1572]
ATGAGCGCAGTTCAGACAAGCAACCTCGCGGGCATTACGGGAATCGCACGCCGCTTGGTGTTGGATGGAGCGCTCGACGA
GGCCACCGCCCGAGACTCGCTAGACAGGGCGACCGCCGAGCGCAAGCCTATTGCGTCATATTTGCGTGAAGCAAAGCTCG
TCACTTCGGCACAAATGGCAGCGGCCAACTCCATTGAATTCGGCATGCCCGTGTTCGACCCCTCGGTCATGGATGGCACG
CAAAGCGCCATCAAGCTGGTCACAGACGAGCTGCTTGCAAAGCACAACGTGCTGCCGCTGTTCAAGCGTGGCGGGCGCCT
GTTCGTCGGCGTCGCAGACCCGACCAATACCCACGCTTTGGACGAGGTCAAGTTCCATACCAATCTGACCGTCGAACCCA
TACTCGTCGACGAAGAGCGCATCCGACGCACCATCGAGCTATGGCAGGAGAGCAGCGATACGTTCGGCGACACACTCGAT
GACAGTGAAGGCTTGGAGAAGCTCGACATCAGCGGGGGTGAGGAGGACGGGACCGACGATGGCGTCAGCGCCAAGGGCGA
CGACACGCCGGTGGTCAAGTTCATCAACAAGGTGCTGATCGACGCCATCAAGAAGGGCGCCTCCGACATCCATTTCGAGC
CGTACGAAACAGACTACCGGGTGCGCCTGCGCATCGACGGCATCCTCAAGCAAGTCGCCAAGATGCCGGTGAAACTCAAC
CAGCGCATCACCGCGCGCTTGAAAGTCATGGCGCAGCTGGACATTGCCGAGAAGCGCGTGCCGCAGGACGGCCGTATCAA
ACTCAACCTCTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACGTTGCCTACGCTGTTCGGCGAGAAGGTCGTGTTGC
GTATCCTCGACGGCAGCGCAGCCAAGCTGGGCGTCGAGAAACTTGGTTACGAGCCTGAGCAGCAGAAGCTGTTCCTGGAA
GCCATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCAACCGGCTCCGGCAAGACGGTGAGCCTGTACACCGCTCT
GGGGATCCTGAACGACGAGACTCGCAATATCTCGACCGCGGAGGACCCGGTGGAAATCCGGTTGCCCGGCGTCAACCAGG
TACAGCAGAACGTAAAGCGCGGCATGACCTTCGCCGCAGCGCTCCGCAGCTTCCTGCGCCAGGACCCGGACATCATCATG
GTGGGTGAGATCCGCGACCTGGAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGCTTAGCACCCT
GCACACCAACGACGCCCCTCAGACCATTGCCCGACTGATGAACATGGGCATTGCGCCCTACAACATCACGAGTTCGGTGA
CCCTGGTAATCGCCCAGCGCTTGGCGCGACGCCTGTGCAGCAAGTGCAAGCAGCCCCAAGAACTGCCAGACAACGCACTT
CTGGCGGAGGGCTTTACCGCCGACGAGATCCATGAAGGATTCACGATCTATGAGGCTGTGGGCTGCGACGAGTGCACCGG
CGGCTACAAGGGACGGCTTGGCGTGTACCAGGTACTGCCGATGAGCGAGGAGATCCAGACCATCGTGCTGCAGGGGGGCA
ATGCGATCCAGATTGCCGAGGTGGCCAAGGCGGCCGGCGTCAAGGACCTGCGTCAATCCGCCCTACTCAAGGTGAAGAAC
GGGCTGACTACACTGGCAGAGATCAACCGGATTACGGTGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.34

100

0.545
  pilB Acinetobacter baylyi ADP1

53.206

100

0.535
  pilB Legionella pneumophila strain ERS1305867

51.852

98.78

0.512
  pilF Neisseria gonorrhoeae MS11

50.667

91.463

0.463
  pilB Vibrio cholerae strain A1552

51.362

89.547

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

48.148

94.077

0.453
  pilB Vibrio campbellii strain DS40M4

52.245

85.366

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

38.909

95.819

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.577

90.592

0.368
  pilF Thermus thermophilus HB27

39.394

91.986

0.362