Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JH287_RS13830 Genome accession   NZ_CP067001
Coordinates   3137362..3137769 (-) Length   135 a.a.
NCBI ID   WP_129588891.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 40-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3138539..3157172 3137362..3137769 flank 770


Gene organization within MGE regions


Location: 3137362..3157172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH287_RS13830 (JH287_13825) pilA/pilAI 3137362..3137769 (-) 408 WP_129588891.1 pilin Machinery gene
  JH287_RS13835 (JH287_13830) pilA/pilAI 3137844..3138254 (-) 411 WP_274497102.1 pilin Machinery gene
  JH287_RS13840 (JH287_13835) pilC 3138614..3139870 (+) 1257 WP_129589289.1 type II secretion system F family protein Machinery gene
  JH287_RS13845 (JH287_13840) - 3139877..3140740 (+) 864 WP_129588889.1 A24 family peptidase -
  JH287_RS13850 (JH287_13845) coaE 3140754..3141374 (+) 621 WP_129588888.1 dephospho-CoA kinase -
  JH287_RS13855 (JH287_13850) - 3141519..3146279 (+) 4761 WP_274503524.1 RHS repeat-associated core domain-containing protein -
  JH287_RS13860 (JH287_13855) - 3146918..3147345 (+) 428 Protein_2674 SymE family type I addiction module toxin -
  JH287_RS13865 (JH287_13860) - 3147421..3147711 (+) 291 WP_014508712.1 DUF1778 domain-containing protein -
  JH287_RS13870 (JH287_13865) - 3147708..3148199 (+) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH287_RS13875 (JH287_13870) - 3148342..3149676 (-) 1335 WP_162808569.1 HAMP domain-containing sensor histidine kinase -
  JH287_RS13880 (JH287_13875) - 3149669..3150346 (-) 678 WP_003490678.1 response regulator transcription factor -
  JH287_RS13885 (JH287_13880) rimK 3150996..3151871 (-) 876 WP_011038222.1 30S ribosomal protein S6--L-glutamate ligase -
  JH287_RS13890 (JH287_13885) glgX 3152364..3154493 (+) 2130 WP_129588886.1 glycogen debranching protein GlgX -
  JH287_RS13895 (JH287_13890) - 3155028..3155420 (-) 393 WP_129588885.1 H-NS family nucleoid-associated regulatory protein -
  JH287_RS13900 (JH287_13895) - 3155511..3155906 (-) 396 WP_016945003.1 hypothetical protein -
  JH287_RS13905 (JH287_13900) - 3156083..3156238 (-) 156 WP_129588884.1 hypothetical protein -
  JH287_RS13910 (JH287_13905) - 3156302..3156532 (+) 231 WP_165349436.1 hypothetical protein -
  JH287_RS13915 (JH287_13910) - 3156822..3157172 (-) 351 WP_274316879.1 hypothetical protein -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14013.19 Da        Isoelectric Point: 8.1079

>NTDB_id=524122 JH287_RS13830 WP_129588891.1 3137362..3137769(-) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 40-2]
MSGQKGFSLIELMIVIAIIAILAAIAFPAYQSSVAKAQLTAALAEIRPGKTTLETVVQDGRDPSLVDKSYVGLTVSARCS
SVSAILDSSGVGEISCTLQGSSLVSGRDLKLKRSAAGLWTCDASAFDQKYRPAGC

Nucleotide


Download         Length: 408 bp        

>NTDB_id=524122 JH287_RS13830 WP_129588891.1 3137362..3137769(-) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 40-2]
ATGTCTGGGCAAAAAGGCTTTTCTCTAATAGAACTGATGATTGTTATAGCGATTATTGCCATATTGGCTGCTATAGCATT
CCCCGCTTATCAAAGTTCAGTTGCAAAAGCGCAGCTTACCGCTGCATTAGCCGAGATAAGGCCAGGGAAAACCACGCTCG
AAACAGTGGTCCAGGATGGTAGGGATCCGTCACTCGTTGATAAAAGTTACGTGGGTTTGACTGTATCCGCGAGGTGTTCC
AGCGTCAGTGCGATTTTGGATTCCTCAGGGGTTGGTGAGATTAGTTGTACCCTCCAAGGATCATCCCTGGTTTCAGGGCG
TGATTTAAAGTTGAAGCGTTCTGCCGCTGGTCTCTGGACCTGTGATGCTTCTGCTTTTGACCAAAAATATCGCCCTGCTG
GTTGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.794

100

0.481
  pilA Acinetobacter baumannii strain A118

43.885

100

0.452
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.481

100

0.415
  pilA Ralstonia pseudosolanacearum GMI1000

33.951

100

0.407
  comP Acinetobacter baylyi ADP1

36.735

100

0.4
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.063

100

0.393
  pilA Vibrio cholerae strain A1552

37.063

100

0.393
  pilA Vibrio cholerae C6706

37.063

100

0.393
  pilE Neisseria gonorrhoeae MS11

32.121

100

0.393
  pilA2 Legionella pneumophila str. Paris

37.5

100

0.378
  pilA2 Legionella pneumophila strain ERS1305867

36.765

100

0.37