Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JH308_RS15065 Genome accession   NZ_CP066979
Coordinates   3412065..3412484 (-) Length   139 a.a.
NCBI ID   WP_116670568.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 55-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3407065..3417484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH308_RS15045 (JH308_15025) pilR 3407248..3408642 (+) 1395 WP_267314854.1 sigma-54 dependent transcriptional regulator Regulator
  JH308_RS15050 (JH308_15030) - 3408840..3409196 (+) 357 WP_139328384.1 hypothetical protein -
  JH308_RS15055 (JH308_15035) - 3409196..3409933 (+) 738 WP_070690009.1 zeta toxin family protein -
  JH308_RS15060 (JH308_15040) pilB 3410116..3411822 (-) 1707 WP_181920283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH308_RS15065 (JH308_15045) pilA2 3412065..3412484 (-) 420 WP_116670568.1 pilin Machinery gene
  JH308_RS15070 (JH308_15050) pilC 3412835..3414094 (+) 1260 WP_070690003.1 type II secretion system F family protein Machinery gene
  JH308_RS15075 (JH308_15055) - 3414101..3414964 (+) 864 WP_267314851.1 A24 family peptidase -
  JH308_RS15080 (JH308_15060) coaE 3414978..3415601 (+) 624 WP_040940796.1 dephospho-CoA kinase -
  JH308_RS15085 (JH308_15065) - 3416133..3416534 (+) 402 WP_267314850.1 SymE family type I addiction module toxin -
  JH308_RS15090 (JH308_15070) - 3416609..3416899 (+) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH308_RS15095 (JH308_15075) - 3416896..3417387 (+) 492 WP_011038220.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14040.23 Da        Isoelectric Point: 9.0169

>NTDB_id=523796 JH308_RS15065 WP_116670568.1 3412065..3412484(-) (pilA2) [Xanthomonas campestris pv. campestris strain 55-2]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLIKARVSEVVLAASSARTTVSEAAAADTGTAMPAAVTIESQASKYVA
SVAYAGGTTNGIITAISKDLGGTATGTITLTGTKSTNGQVAWVCGGTIPAKYRPATCQG

Nucleotide


Download         Length: 420 bp        

>NTDB_id=523796 JH308_RS15065 WP_116670568.1 3412065..3412484(-) (pilA2) [Xanthomonas campestris pv. campestris strain 55-2]
ATGAAGAAGCAACAAGGTTTTACCCTGATTGAATTGATGATCGTCGTGGCCATCATTGCCATTCTTGCCGCTATCGCGCT
GCCGGCCTATCAGGATTACCTGATCAAGGCTCGCGTATCCGAGGTGGTTCTGGCTGCATCTTCCGCGCGTACTACAGTTT
CTGAAGCCGCGGCGGCTGACACTGGTACGGCCATGCCTGCCGCAGTTACGATTGAGAGCCAGGCGTCCAAGTACGTAGCA
TCTGTTGCTTATGCCGGCGGCACCACAAATGGTATTATCACTGCAATCTCCAAGGATCTTGGCGGTACTGCTACTGGAAC
GATTACGTTGACTGGTACCAAGTCTACTAATGGACAGGTCGCCTGGGTCTGTGGCGGCACGATCCCGGCTAAGTATCGTC
CGGCAACTTGCCAGGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.817

100

0.54
  pilA2 Legionella pneumophila str. Paris

53.191

100

0.54
  comP Acinetobacter baylyi ADP1

46.98

100

0.504
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.043

100

0.504
  pilA Ralstonia pseudosolanacearum GMI1000

40.491

100

0.475
  pilA/pilA1 Eikenella corrodens VA1

40.132

100

0.439
  pilE Neisseria gonorrhoeae MS11

36.25

100

0.417
  pilE Neisseria gonorrhoeae strain FA1090

35.404

100

0.41
  pilA Vibrio parahaemolyticus RIMD 2210633

39.13

99.281

0.388
  pilA Acinetobacter baumannii strain A118

37.857

100

0.381