Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   JH274_RS04945 Genome accession   NZ_CP066968
Coordinates   1157763..1158188 (+) Length   141 a.a.
NCBI ID   WP_274395997.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. incanae strain 16-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1152763..1163188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH274_RS04915 (JH274_04905) - 1152855..1153346 (-) 492 WP_274395994.1 GNAT family N-acetyltransferase -
  JH274_RS04920 (JH274_04910) - 1153343..1153633 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH274_RS04925 (JH274_04915) - 1153709..1154110 (-) 402 WP_274395995.1 SymE family type I addiction module toxin -
  JH274_RS04930 (JH274_04920) coaE 1154642..1155265 (-) 624 WP_116670565.1 dephospho-CoA kinase -
  JH274_RS04935 (JH274_04925) - 1155279..1156142 (-) 864 WP_040940797.1 A24 family peptidase -
  JH274_RS04940 (JH274_04930) pilC 1156149..1157408 (-) 1260 WP_274395996.1 type II secretion system F family protein Machinery gene
  JH274_RS04945 (JH274_04935) pilA2 1157763..1158188 (+) 426 WP_274395997.1 pilin Machinery gene
  JH274_RS04950 (JH274_04940) - 1158324..1159916 (+) 1593 WP_337998769.1 phosphoethanolamine transferase -
  JH274_RS04955 (JH274_04945) - 1159927..1160556 (+) 630 WP_012437604.1 hypothetical protein -
  JH274_RS04960 (JH274_04950) - 1160526..1162052 (+) 1527 WP_337998762.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14496.77 Da        Isoelectric Point: 9.0169

>NTDB_id=523677 JH274_RS04945 WP_274395997.1 1157763..1158188(+) (pilA2) [Xanthomonas campestris pv. incanae strain 16-8]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGLAQVTAANSGYAFPGATK
YVSGVAITNTTGRVTVTSTVPNAIGDILLTPTDVGGGQLKWTCSSAINVKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=523677 JH274_RS04945 WP_274395997.1 1157763..1158188(+) (pilA2) [Xanthomonas campestris pv. incanae strain 16-8]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTTGTGGCAATCATTGCCATCCTGGCGGCAATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGCGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAGCTTGCGGTCA
CGGAGACTGCATCGTCGCTCGGCGGCCTCGCTCAGGTTACTGCGGCAAATTCGGGCTACGCATTCCCGGGTGCCACGAAG
TATGTCAGCGGCGTCGCTATCACCAATACCACGGGTCGCGTTACGGTGACCTCCACGGTGCCCAACGCTATCGGTGACAT
CCTGCTGACCCCGACTGATGTTGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCAACGTCAAGTATCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.206

96.454

0.504
  pilA2 Legionella pneumophila str. Paris

52.206

96.454

0.504
  pilA Ralstonia pseudosolanacearum GMI1000

40.244

100

0.468
  pilE Neisseria gonorrhoeae MS11

40.123

100

0.461
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.595

100

0.454
  comP Acinetobacter baylyi ADP1

42.857

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

38.608

100

0.433
  pilA/pilA1 Eikenella corrodens VA1

39.333

100

0.418
  pilA Pseudomonas aeruginosa PAK

37.013

100

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.176

96.454

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.481

95.745

0.397
  pilA Acinetobacter baumannii strain A118

35.172

100

0.362