Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JH280_RS03505 Genome accession   NZ_CP066922
Coordinates   800653..801786 (-) Length   377 a.a.
NCBI ID   WP_274348249.1    Uniprot ID   -
Organism   Xanthomonas campestris strain 5053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795653..806786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH280_RS03480 (JH280_03480) - 795677..796012 (-) 336 WP_115000044.1 phasin family protein -
  JH280_RS03485 (JH280_03485) queD 796202..796555 (-) 354 WP_274348248.1 6-carboxytetrahydropterin synthase QueD -
  JH280_RS03490 (JH280_03490) - 796670..798058 (-) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  JH280_RS03495 (JH280_03495) - 798438..799427 (+) 990 WP_068573758.1 fumarylacetoacetate hydrolase family protein -
  JH280_RS03500 (JH280_03500) maiA 799471..800133 (+) 663 WP_103701385.1 maleylacetoacetate isomerase -
  JH280_RS03505 (JH280_03505) pilU 800653..801786 (-) 1134 WP_274348249.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JH280_RS03510 (JH280_03510) - 801885..802268 (+) 384 WP_011035838.1 DUF4398 domain-containing protein -
  JH280_RS03515 (JH280_03515) - 802265..803155 (+) 891 WP_076036459.1 hypothetical protein -
  JH280_RS03520 (JH280_03520) - 803496..803714 (+) 219 WP_019237985.1 YdcH family protein -
  JH280_RS03525 (JH280_03525) - 803946..805316 (+) 1371 WP_115539586.1 pyridoxal-phosphate dependent enzyme -
  JH280_RS03530 (JH280_03530) - 805410..806606 (+) 1197 WP_274348250.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41814.08 Da        Isoelectric Point: 6.1401

>NTDB_id=523195 JH280_RS03505 WP_274348249.1 800653..801786(-) (pilU) [Xanthomonas campestris strain 5053]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGQDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=523195 JH280_RS03505 WP_274348249.1 800653..801786(-) (pilU) [Xanthomonas campestris strain 5053]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAAGTGGGCATGGTGATCCGTGCTATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACTGGCT
CGGGCAAGTCGACCTCGCTGGCCTCAATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATT
CTAAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATTTGCGTGCGGTGGTCTCCCAGCG
CCTGGTCAAGGGCCAGGACGGACGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTAGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363