NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	I6G50_RS09580	Genome accession	NZ_CP065691
Coordinates	1910854..1911789 (+)	Length	311 a.a.
NCBI ID	WP_197908696.1	Uniprot ID	-
Organism	Lactococcus garvieae strain FDAARGOS_893
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1905854..1916789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G50_RS09565 (I6G50_09560)	-	1907334..1908785 (-)	1452	WP_003134541.1	GH25 family lysozyme	-
I6G50_RS09570 (I6G50_09565)	guaB	1908960..1910441 (+)	1482	WP_003134540.1	IMP dehydrogenase	-
I6G50_RS09575 (I6G50_09570)	rpsU	1910567..1910743 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
I6G50_RS09580 (I6G50_09575)	comGA	1910854..1911789 (+)	936	WP_197908696.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G50_RS09585 (I6G50_09580)	comGB	1911737..1912756 (+)	1020	WP_269668697.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G50_RS09590 (I6G50_09585)	comYC	1912779..1913084 (+)	306	WP_197908697.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G50_RS09595 (I6G50_09590)	comGD	1913059..1913475 (+)	417	WP_042753018.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G50_RS09600 (I6G50_09595)	comGE	1913507..1913740 (+)	234	WP_003134534.1	competence type IV pilus minor pilin ComGE	-
I6G50_RS09605 (I6G50_09600)	comGF	1913745..1914155 (+)	411	WP_232252357.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G50_RS09610 (I6G50_09605)	-	1914394..1915239 (+)	846	WP_197908698.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
I6G50_RS09615 (I6G50_09610)	-	1915323..1916192 (-)	870	WP_197908699.1	RluA family pseudouridine synthase	-

Sequence

Protein

Download Length: 311 a.a. Molecular weight: 35306.62 Da Isoelectric Point: 8.8723

                                >NTDB_id=513025 I6G50_RS09580 WP_197908696.1 1910854..1911789(+) (comGA) [Lactococcus garvieae strain FDAARGOS_893]

MIQKLARDLLQKAVEKSIHDIYLIAILGSYRLYFRTASERKFEQTLPLEQGQSLIAHLKFISGMNLGETRRTQLGSCSYP

LAGVEQRLRLSTVGDFEGNESLVIRLLHNGKDKLSFWQKDIYNNLIEGRGLYLFSGPVGSGKTSLMYECARRHFQEQQVI

CIEDPVELVEPHFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDKQTAKAVLRASLTGYTVFSTVHAKSIPGVVAR

LKELGLTDWELQSSLQLVAYQRLIAGKGLLVYAKKEFESWQPENWNKQINQLFTDGYISAATASYEKIESN

Nucleotide

Download Length: 936 bp

                                >NTDB_id=513025 I6G50_RS09580 WP_197908696.1 1910854..1911789(+) (comGA) [Lactococcus garvieae strain FDAARGOS_893]

ATGATTCAAAAATTGGCAAGGGATCTTTTGCAAAAAGCAGTAGAAAAAAGTATTCATGATATTTATCTGATTGCTATCTT

AGGAAGTTACCGACTTTATTTCCGTACCGCAAGTGAGAGGAAATTTGAGCAAACCCTCCCTCTAGAACAAGGGCAATCCT

TGATTGCACATCTAAAATTTATCTCGGGGATGAATCTTGGCGAAACGAGACGAACACAGCTTGGTTCTTGTTCTTACCCG

CTTGCGGGAGTCGAGCAGCGTTTGCGCCTTTCCACTGTTGGTGATTTCGAAGGAAATGAAAGTCTGGTTATCCGTCTTCT

CCATAATGGGAAAGATAAGTTGAGCTTTTGGCAAAAGGATATCTATAATAATCTTATAGAAGGAAGGGGACTCTATCTTT

TTTCTGGACCCGTTGGTTCAGGGAAAACCAGTTTAATGTATGAATGTGCTCGGCGCCATTTTCAAGAGCAACAGGTGATT

TGTATTGAAGATCCAGTGGAGCTAGTGGAACCCCATTTTTTACAGCTTCAAGTGAACAAGGTGATTGGAAATGATTATGA

TGCCTTGATTAAGCTCTCTTTGCGTCATCGCCCCGATTTGCTGATTGTTGGAGAAATAAGAGATAAGCAGACGGCTAAGG

CCGTTTTACGTGCGAGTTTAACAGGTTATACCGTTTTTTCTACTGTACATGCAAAGTCAATCCCCGGTGTAGTTGCTCGC

CTGAAAGAACTAGGCTTAACAGATTGGGAATTACAATCAAGTCTTCAGCTGGTGGCTTACCAACGCTTGATTGCAGGAAA

AGGACTATTGGTTTATGCAAAAAAAGAATTCGAAAGCTGGCAGCCAGAAAACTGGAATAAACAAATTAACCAACTTTTTA

CAGATGGATATATCTCAGCTGCTACAGCTTCGTACGAAAAAATTGAGTCTAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	60.897	100	0.611
comYA	Streptococcus mutans UA159	55.272	100	0.556
comYA	Streptococcus mutans UA140	55.272	100	0.556
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	55.272	100	0.556
comYA	Streptococcus gordonii str. Challis substr. CH1	52.716	100	0.531
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.226	99.678	0.531
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.581	99.678	0.524
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.581	99.678	0.524
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.581	99.678	0.524
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.581	99.678	0.524

Detailed information NTG0513025